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Caliper labchip ez reader

Manufactured by PerkinElmer
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The Caliper LabChip EZ Reader is a laboratory instrument designed for automated analysis of DNA, RNA, and proteins. It utilizes microfluidic technology to perform electrophoretic separation and detection of samples in a highly efficient and rapid manner.

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Lab products found in correlation

Aurkb, by Merck Group (2 mentions)

3 protocols using caliper labchip ez reader

1

Enzyme Kinetic Assay for AURKB Inhibition

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Enzyme kinetic experiments were performed at pH 7.0 in 8 mM MOPS buffer with 0.2 mM EDTA and 10 mM magnesium acetate. Reactions were assembled in 384-well plate wells by adding 400 ng/ml of AURKB (EMD Millipore cat. no. 14-835) into separate reaction mixtures containing 1.5μM fluorescently labeled Caliper peptide substrate (FL-peptide 1, 5-FAM-AKRRRLSSLRA-COOH, Perkin-Elmer cat. no. 760345) with various concentrations of ATP and compound (BRD-7880, tozasertib, barasertib). The final ATP concentrations varied from 6.25 to 200 μM and compound varied from 0 to 200 nM. Plates were immediately placed into a Perkin-Elmer Caliper LabChip EZ Reader and wells were sampled periodically throughout a 1-hour reaction period for initial reaction rate. The fluorescent product and substrate were separated and monitored on the Caliper microfluidic instrument. The conversion of substrate was calculated with Caliper software. Km and ki values were determined from the double reciprocal Lineweaver-Burk plot by linear regression with GraFit 6 software (Erithacus Software Ltd., Horley, U.K.) using competitive inhibition equation modeling.
Yu C., Mannan A.M., Yvone G.M., Ross K.N., Zhang Y.L., Marton M.A., Taylor B.R., Crenshaw A., Gould J.Z., Tamayo P., Weir B.A., Tsherniak A., Wong B., Garraway L.A., Shamji A.F., Palmer M.A., Foley M.A., Winckler W., Schreiber S.L., Kung A.L, & Golub T.R. (2016). High-throughput identification of genotype-specific cancer vulnerabilities in mixtures of barcoded tumor cell lines. Nature biotechnology, 34(4), 419-423.
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2

Kinase Assay for HPK1 Inhibitor Evaluation

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The kinase assay carried out in 20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) pH 7.5, 10 mM MgCl2, 0.015% Brij-15 and 2 mM Dithiothreitol (DTT) containing 0.625 nM recombinant HPK1 (SingalChem M23-11G), 3 µM fluorescently labeled peptide substrate (NH2-fluorescein-RFARKGSLRQKNV-COOH), 22 µM ATP, and various concentrations of HPK1 inhibitor. The reactions were incubated for 3 hours and quenched with 1 mM EDTA solution, followed by capillary electrophoresis on a Caliper LabChip EZ Reader (PerkinElmer) to determine peptide substrate conversion and IC50 values.
You D., Hillerman S., Locke G., Chaudhry C., Stromko C., Murtaza A., Fan Y., Koenitzer J., Chen Y., Briceno S., Bhadra R., Duperret E., Gullo-Brown J., Gao C., Zhao D., Feder J., Curtin J., Degnan A.P., Kumi G., Wittman M., Johnson B.M., Parrish K.E., Gokulrangan G., Morrison J., Quigley M., Hunt J.T., Salter-Cid L., Lees E., Sanjuan M.A, & Liu J. (2021). Enhanced antitumor immunity by a novel small molecule HPK1 inhibitor. Journal for Immunotherapy of Cancer, 9(1), e001402.
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3

Enzyme Kinetic Assay for AURKB Inhibition

Check if the same lab product or an alternative is used in the 5 most similar protocols
Enzyme kinetic experiments were performed at pH 7.0 in 8 mM MOPS buffer with 0.2 mM EDTA and 10 mM magnesium acetate. Reactions were assembled in 384-well plate wells by adding 400 ng/ml of AURKB (EMD Millipore cat. no. 14-835) into separate reaction mixtures containing 1.5μM fluorescently labeled Caliper peptide substrate (FL-peptide 1, 5-FAM-AKRRRLSSLRA-COOH, Perkin-Elmer cat. no. 760345) with various concentrations of ATP and compound (BRD-7880, tozasertib, barasertib). The final ATP concentrations varied from 6.25 to 200 μM and compound varied from 0 to 200 nM. Plates were immediately placed into a Perkin-Elmer Caliper LabChip EZ Reader and wells were sampled periodically throughout a 1-hour reaction period for initial reaction rate. The fluorescent product and substrate were separated and monitored on the Caliper microfluidic instrument. The conversion of substrate was calculated with Caliper software. Km and ki values were determined from the double reciprocal Lineweaver-Burk plot by linear regression with GraFit 6 software (Erithacus Software Ltd., Horley, U.K.) using competitive inhibition equation modeling.
Yu C., Mannan A.M., Yvone G.M., Ross K.N., Zhang Y.L., Marton M.A., Taylor B.R., Crenshaw A., Gould J.Z., Tamayo P., Weir B.A., Tsherniak A., Wong B., Garraway L.A., Shamji A.F., Palmer M.A., Foley M.A., Winckler W., Schreiber S.L., Kung A.L, & Golub T.R. (2016). High-throughput identification of genotype-specific cancer vulnerabilities in mixtures of barcoded tumor cell lines. Nature biotechnology, 34(4), 419-423.
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