Sybr green pcr master mix 2

TC treated cell culture dishes (Cat. F611203) and noTC treated polystyrene culture dishes (Cat. F611003) were purchased from Sangon Biotech (Shanghai). SYBR Green PCR Master Mix (2×) (Cat. 4913914001) was from Roche. RNAiso Plus (Cat. 9109) and PrimeScript™ RT reagent kit (Cat. RR047A) with gDNA eraser were from TaKaRa. LPS (Cat. L2630) and accutase solution containing 0.5 mM EDTA (Cat. A6964) were obtained from Sigma-Aldrich. 0.5 M EDTA was obtained from Solarbio (Cat. 420404). 0.25% Trypsin containing 0.02% EDTA was from Zhejiang Senrui Biological Technology (Cat.CR-25200). Recombinant mouse M-CSF (Cat. 415-ML) and GM-CSF (Cat. 416-ML) were purchased from R&D Systems. Debris Removal Solution (Cat. 130-110-203) and antibodies against Ly6C (APC) (Cat. 130-111-779) were from Miltenyi Biotec. 7-AAD Viability Staining Solution (Cat. 51-68981E) and antibodies against F4/80 (PE) (Cat. 565410) were from BD Bioscience. Anti-MHCII (Brilliant Violet 421) (Cat. 107632) antibodies were from Biolegend. Antibodies against CD11b (PE-CY7) (Cat. 25-0112-82) and CD16/CD32 (Cat. 14-0161-86) and Rat IgG1 kappa isotype control (Cat. 14-4301-85) were from Invitrogen.

Primary antibodies used in the study were detailed in Table S1. Cell lysis buffer (Cat. 9803) was purchased from Cell Signaling Technology. Protease Inhibitor (Cat. 11836145001), Phosphorylation Inhibitor Cocktail Reagent (Cat. 4906837001) and SYBR Green PCR Master Mix (2×) (Cat. 4913914001) were from Roche Diagnostics. Horseradish peroxidase-conjugated secondary antibodies (Cat. abs20001; abs20002) were obtained from Absin. Kolliphor^® HS 15 (HS-15) (Cat. 42966) and lipopolysaccharide/LPS (Cat. 916374) were from Sigma. Recombinant mouse IL-6 (Cat. 200-06), human and mouse TNF-α (Cat. 315-01A; 300-01A), mouse IFN-γ (Cat. 315-05) and IFN-β (Cat. 300-02BC) were obtained from PeproTech. Mouse granulocyte colony-stimulating factor (G-CSF) ELISA Kit (Cat. ab197743) was bought from Abcam. Mouse IL-6 ELISA Kit (Cat. abs520004) and Mouse IL-1β ELISA Kit (Cat. abs520001) were purchased from Absin. Urea Assay Kit (Cat. C013-2-1), Alanine aminotransferase (ALT) Assay Kit (Cat. C009-2-1), and Aspartate aminotransferase (AST) Assay Kit (Cat. C010-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute. The detailed compound library information was as in our previously published literature (14 (link)). The bioactive compound C498-0670 (C498) was acquired from TargetMol.

TIANamp Genomic DNA Kit (Cat. DP304) was from Tiangen. SYBR Green PCR Master Mix (2×) (Cat.4913914001) was from Roche. Recombinant mouse M-CSF (Cat. 415-ML) and GM-CSF (Cat. 416-ML) were purchased from R&D Systems. Glycolytic Rate Assay Kit (Cat. 103346-100), XF cell Mito Stress Test Kit (Cat. 103010-100) and Cell Energy Phenotype Test Kit (Cat. 103275-100) were from Agilent. Glucose Assay Kit (Cat. AB169559), Citrulline Fluorometric Assay Kit (Cat. AB273309), Ornithine Fluorometric Assay Kit (Cat. AB252903), and Adipogenesis Colorimetric/Fluorometric Assay Kit (Cat. AB102513) were form Abcam. Nitric Oxide Assay Kit (Cat. S0021S), Nitric Oxide Synthase Assay Kit (Cat. S0025) and Mito-Tracker Green (Cat. C1048) was from Beyotime. Bis Benzimide Hoechst NO33342 (Cat. B8040) was from Solarbio. Arginase Activity Assay Kit (Cat. MAK112) was from Sigma-Aldrich.