Wild-type BJ1991α (MAT α, leu2, trp1, ura3-52, pep4-3, prb1-1122, gal2)48 (link) and BJ1991α Δetr1 (MAT α, leu2, trp1, ura3-52, pep4-3, prb1-1122, gal2; ybr026c::kanMX4)14 (link) mutant strains of S. cerevisiae are used in this study. Media used are as follows: YPD [1% yeast extract (DIFCO, NJ, USA), 2% Bacto-peptone (DIFCO), 2% D-glucose], SCD [0.67% Yeast Nitrogen Base without amino acids (DIFCO), 0.19% Synthetic complete drop out mix without uracil (Sigma-Aldrich, St. Louis, MO, USA), 0.008% uracil, 2% D-glucose], SCG [0.67% Yeast Nitrogen Base without amino acids (DIFCO), 0.19% Synthetic complete drop out mix without uracil (Sigma-Aldrich), 0.008% uracil, 3% glycerol], SCD/-Uracil [0.67% Yeast Nitrogen Base without amino acids (DIFCO), 0.19% Synthetic complete drop out mix without uracil (Sigma-Aldrich), 2% D-glucose], SCG/-Uracil [0.67% Yeast Nitrogen Base without amino acids (DIFCO), 0.19% Synthetic complete drop out mix without uracil (Sigma-Aldrich), 3% glycerol]. Solid media was prepared with 2% Agar (Fisher BioReagentsTM, Geel, Belgium).
Tanvir Rahman M., Kristian Koski M., Panecka-Hofman J., Schmitz W., Kastaniotis A.J., Wade R.C., Wierenga R.K., Kalervo Hiltunen J, & Autio K.J. (2023). An engineered variant of MECR reductase reveals indispensability of long-chain acyl-ACPs for mitochondrial respiration. Nature Communications, 14, 619.
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