Cf 573 couette flow cell unit

LD experiments (Nordén et al., 2010 ) were run using a Jasco J-810 Circular Dichroism SpectroPolarimeter fitted with a CF-573 Couette flow cell unit. The cell used has a pathlength of 1 mm (2 × 0.5 mm) and a 100 μL volume; the Couette cell consisted of an outer quartz cylinder and an inner quartz rod. A shear gradient was generated by the rotation of the inner quartz glass rod, and the DNA alignment is at a tangent to the direction of flow. The LD spectra of all samples were measured at 4,080 rpm to give the maximum LD signal and also at 0 rpm to give a background. All samples were subsequently zeroed and baselined by subtracting LD at 0 rpm from that at 4,080 rpm. A scan speed of 100 nm/min, response time of 0.5 s, 0.5 nm step, and 1 nm bandwidth were used.

LD experiments (Nordén et al., 2010 ) were run using a Jasco J-810 Circular Dichroism SpectroPolarimeter fitted with a CF-573 Couette flow cell unit. The cell used has a pathlength of 1 mm (2 × 0.5 mm) and a 100 μL volume; the Couette cell consisted of an outer quartz cylinder and an inner quartz rod. A shear gradient was generated by the rotation of the inner quartz glass rod, and the DNA alignment is at a tangent to the direction of flow. The LD spectra of all samples were measured at 4,080 rpm to give the maximum LD signal and also at 0 rpm to give a background. All samples were subsequently zeroed and baselined by subtracting LD at 0 rpm from that at 4,080 rpm. A scan speed of 100 nm/min, response time of 0.5 s, 0.5 nm step, and 1 nm bandwidth were used.