The phenotype of cells from mouse lungs was determined by incubating lung leukocytes with the indicated antibodies and CD16/32 to limit nonspecific binding. Leukocytes were stained at 4°C for 15 min in PBS containing 1%BSA and 0.01% sodium azide. Cells were stained with combinations of the following antibodies: FITC-conjugated I-Ab; PE-conjugated CD11c; PerCP-conjugated CD45; and APC-conjugated F4/80 and EpCAM from BD Biosciences. For intracellular IL-33 staining, cells were incubated with Cytofix/Cytoperm (BD Biosciences, San Diego, CA), washed in Permeabilization Buffer (BD Biosciences), and stained for 30 min with PE-conjugated IL-33 (R&D systems, Minneapolis, MN). Cells were washed and resuspended in 1% paraformaldehyde. Isotype controls were used. Data was acquired using BD Accuri™ C6 cytometer and analyzed using FCS Express 4.0 Software. For cell sorting experiments, F4/80+ leukocytes from the lungs of WT and CCR2−/− mice were isolated at day-7 p.i. using 5-laser FACS Aria II (BD Biosciences).
Pe conjugated cd11c
Manufactured by BD
PE-conjugated CD11c is a cell surface marker that is commonly used to identify dendritic cells in flow cytometry applications. It is a transmembrane protein that is expressed on the surface of myeloid dendritic cells and plays a role in antigen presentation.
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Lab products found in correlation
Permeabilization buffer, by BD (2 mentions)
Fitc conjugated 1 ab, by BD (2 mentions)
Apc conjugated f4 80 and epcam, by BD (2 mentions)
Pe conjugated il 33, by R&D Systems (2 mentions)
5 laser facs aria 2, by BD (2 mentions)
Percp conjugated cd45, by BD (2 mentions)
Accuri c6 cytometer, by BD (2 mentions)
Cytofix cytoperm, by BD (2 mentions)
2 protocols using pe conjugated cd11c
1
Lung Leukocyte Phenotyping in Mice
2
Lung Leukocyte Phenotyping in Mice
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The phenotype of cells from mouse lungs was determined by incubating lung leukocytes with the indicated antibodies and CD16/32 to limit nonspecific binding. Leukocytes were stained at 4°C for 15 min in PBS containing 1%BSA and 0.01% sodium azide. Cells were stained with combinations of the following antibodies: FITC-conjugated I-Ab; PE-conjugated CD11c; PerCP-conjugated CD45; and APC-conjugated F4/80 and EpCAM from BD Biosciences. For intracellular IL-33 staining, cells were incubated with Cytofix/Cytoperm (BD Biosciences, San Diego, CA), washed in Permeabilization Buffer (BD Biosciences), and stained for 30 min with PE-conjugated IL-33 (R&D systems, Minneapolis, MN). Cells were washed and resuspended in 1% paraformaldehyde. Isotype controls were used. Data was acquired using BD Accuri™ C6 cytometer and analyzed using FCS Express 4.0 Software. For cell sorting experiments, F4/80+ leukocytes from the lungs of WT and CCR2−/− mice were isolated at day-7 p.i. using 5-laser FACS Aria II (BD Biosciences).
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