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Il 18bp

Manufactured by RayBiotech
Sourced in Japan

IL-18BP is a laboratory product manufactured by RayBiotech. It is a recombinant protein that functions as an interleukin-18 binding protein.

Automatically generated - may contain errors

2 protocols using il 18bp

1

Antibody Sourcing for Protein Analysis

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β-Actin antibody was purchased from Santa Cruz Biotechnology (Dallas, Texas, USA), and HRP-conjugated rabbit anti-human IgG was purchased from Jackson ImmunoResearch (West Grove, PA, USA); ICAM-1 and IL-18 antibody were purchased from Abcam (Cambridge, MA, USA). IL-37 was purchased from Adipogen AG, Liestal, Switzerland, IL-18 from MBL, Nagoya, Japan, IL-18BP from RayBiotech, Norcross GA, USA, and hs-CRP from Elisa Biotech (Shanghai, China).
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2

Plasma Cytokine Quantification by ELISA

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The levels of plasma IL-37 (Adipogen AG, Liestal, Switzerland), IL-18 (MBL, Nagoya, Japan), and IL-18BP (RayBiotech, Norcross GA, USA) were measured by an enzyme-linked immunosorbent assay (ELISA), following the manufacturer's instructions. The minimal detectable concentrations were 10 pg/mL for IL-37, 12.5 pg/mL for IL-18, and 20 pg/mL for IL-18BP. The ELISA intra-assay and interassay coefficients of variation were <5% and <10%, respectively. All of the samples were measured in duplicate.
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