Infinium human cytosnp 850k v1.2 beadchip

Genomic DNA of unedited and edited clones of THP-1 and Jurkat cell lines, and patient-derived LCLs was used for SNP-array copy number profiling and analysis of regions of homozygosity with the Infinium Human CytoSNP-850K v1.2 BeadChip (Illumina, San Diego, CA, USA). This array has ∼850,000 single nucleotide polymorphisms (SNPs) markers across the genome and can detect genomic insertions and deletions as well as stretches of homozygosity. Data analysis was conducted using NxClinical software v6.0 (Bionano genomics, San Diego, CA, USA). Human genome build Feb. 2009 GRCh37/hg19 was used. Results were classified with BENCH Lab CNV software (Agilent, Santa Clara, CA, USA). The genotype data were used for cell line verification and monitoring for potential genomic alterations in single cell cultures.