Liproxstatin 1

Manufactured by Bio-Techne

Sourced in United States

Liproxstatin-1 is a small molecule that functions as a potent and specific inhibitor of ferroptosis, a form of regulated cell death. It acts by blocking the activity of lipid peroxidases, thereby preventing the accumulation of oxidized lipids that trigger ferroptosis. Liproxstatin-1 is commonly used in research applications to study the mechanisms and pathways involved in ferroptosis.

Automatically generated - may contain errors

Lab products found in correlation

Acetonitrile, by Thermo Fisher Scientific (1 mentions) N hexane, by Thermo Fisher Scientific (1 mentions) Sodium borohydride, by Thermo Fisher Scientific (1 mentions) 2 propanol, by Thermo Fisher Scientific (1 mentions) 5s r hete, by Cayman Chemical (1 mentions) 12s r hete, by Cayman Chemical (1 mentions) 15s r hete, by Cayman Chemical (1 mentions) Phosphate buffered saline (pbs), by PAN Biotech (1 mentions) Hplc grade methanol, by Thermo Fisher Scientific (1 mentions) Acetic acid, by Carl Roth (1 mentions) Deferoxamine mesylate, by Merck Group (1 mentions) Triacsin c, by Cayman Chemical (1 mentions) Baicalein, by Bio-Techne (1 mentions) Celltiter 96 aqueous cell proliferation assay kit, by Promega (1 mentions) Z vad fmk, by Apexbio (1 mentions) Fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions) Spectramax id5, by Molecular Devices (1 mentions) Ferrostatin 1, by Merck Group (1 mentions)

2 protocols using liproxstatin 1

Lipid Analysis of SH-SY5Y Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The chemicals used were purchased from the following vendors: SH-SY5Y cells from the German Collection of Microorganisms and Cell Culture GmbH (DSMZ, Braunschweig, Germany); phosphate-buffered saline (PBS) from PAN Biotech (Aidenbach, Germany); cell culture materials and media from PAN Biotech (Aidenbach, Germany), authentic HPLC standards (1 5S/R-HETE, 12S/R-HETE, 5S/R-HETE) from Cayman Chem (distributed by Biomol GmbH, Hamburg, Germany); acetic acid from Carl Roth GmbH (Karlsruhe, Germany); sodium borohydride from Life Technologies, Inc. (Eggenstein, Germany); butylhydroxy toluene (BHT) from European Pharmacopoeia (Strasbourg, France). RSL3 and Ferrostatin-1 from Cayman (Ann Arbor, MI, USA), ML162 and Liproxstatin-1 from Tocris Bioscience (Bristol, UK). Oligonucleotide synthesis was performed at BioTez Berlin Buch GmbH (Berlin, Germany). HPLC-grade methanol, acetonitrile, n-hexane, 2-propanol, and water were from Fisher Scientific (Newington, NH, USA).
For HPLC analysis of the cellular lipids, a Shimadzu instrument (Shimadzu Germany, Berlin, Germany) involving two LC-20 AD pumps, a DGU-20A3 degassing unit, an SPD-M20A diode array detector, a CTO-20 AC column oven, a CBM-20A steering unit, and a SIL-20AC auto-injector were used.

Faraji P., Borchert A., Ahmadian S, & Kuhn H. (2024). Butylated Hydroxytoluene (BHT) Protects SH-SY5Y Neuroblastoma Cells from Ferroptotic Cell Death: Insights from In Vitro and In Vivo Studies. Antioxidants, 13(2), 242.

+ Open protocol

+ Expand

UV-Induced Cell Viability Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK and melanocytes were plated in 96-wells (1 × 10 [4 (link)] cells/well) 12–16 h at 37C with ferrostatin-1 (Sigma, 10 μM), liproxstatin-1 (Tocris, 1 μM), baicalein (Tocris, 5 μM), triacsin C (Cayman Chem, 10 μM), z-VAD-fmk (Apexbio, 20 μM), deferoxamine mesylate (Sigma, 5 and 10 μM). Plates were exposed to UVA (20 kJ/m²) or UVB (2–10 kJ/m²), incubated 6 h at 37C, and viability was assessed with CellTiter 96 Aqueous Cell Proliferation Assay kit (MTS, Promega) according to manufacturer protocol, by 490 nm absorbance measured with SpectraMax iD5 (Molecular Devices) microplate reader. Alternatively, Fixable Viability Dye eFluor 780 (Invitrogen) was used to assess viability by flow cytometry at 6 h, 24 h and 48 h after irradiation.

Vats K., Kruglov O., Mizes A., Samovich S.N., Amoscato A.A., Tyurin V.A., Tyurina Y.Y., Kagan V.E, & Bunimovich Y.L. (2021). Keratinocyte death by ferroptosis initiates skin inflammation after UVB exposure. Redox Biology, 47, 102143.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!