Human metastatic melanoma tissues were screened for Wnt6 and Wnt10b protein expression by immunohistochemistry. Sampling and handling of patient material was carried out in accordance with the ethical principles of the Declaration of Helsinki. The use of human tissue material had been approved by the local ethics committee of the University of Regensburg (application numbers 09/11 and 03/151). The samples were prepared as described previously [40 (link)]. Standard 5 μm sections of formalin‐fixed and paraffin‐embedded tissue blocks were used for immunohistochemical analysis of human tissue samples (tissue microarray (TMA) comprising specimens from benign nevi, primary melanoma and melanoma metastases) [41 ]. Immunohistochemical staining were performed using anti-Wnt6 antibody (Abcam, Cambridge, UK; ab50030 1:100) or anti-Wnt10b antibody (Abcam, Cambridge, UK; ab70816 1:100).
Ab50030
Manufactured by Abcam
Sourced in United Kingdom
Ab50030 is a laboratory equipment product. It is designed to perform a specific function in a laboratory setting. However, without more detailed information about the product, I cannot provide a description that is sufficiently factual and unbiased while maintaining conciseness. As a Marketing Specialist, I aim to present accurate and objective information to our customers, and in this case, a detailed description that meets those criteria is not available.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using ab50030
1
Immunohistochemical Analysis of Wnt Proteins in Metastatic Melanoma
2
Western Blot Analysis of Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blotting was carried out using standard protocols. The primary antibodies used were as follows: KIF26B (1:500, 17422-1-AP, Proteintech), ELK1 (1:500, ab32106, Abcam), Wnt6 (1:1,500, ab50030, Abcam), GAPDH (1:1,000, #5174, CST), β-catenin (1:2,000, ab32532, Abcam), c-Myc (1:1,000, #18583, CST), cyclin D1 (1:1,000, #55506, CST), CDK4 (1:1,000, #12790, CST), p27 (1:1,000, #3686, CST) cyclin E1 (1:1,000, #20808, CST), CDK2 (1:1,000, #18048, CST), p21 (1:1,000, #2947, CST, CDK6 (1:1,000, #13331, CST), p53 (1:1,000, #9282, CST), p-Rb (1:1,000, #8516, CST). Each experiment was performed in triplicate.
3
Western Blot Analysis of Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blotting was carried out using standard protocols. The primary antibodies used were as follows: KIF26B (1:500, 17422-1-AP, Proteintech), ELK1 (1:500, ab32106, Abcam), Wnt6 (1:1,500, ab50030, Abcam), GAPDH (1:1,000, #5174, CST), β-catenin (1:2,000, ab32532, Abcam), c-Myc (1:1,000, #18583, CST), cyclin D1 (1:1,000, #55506, CST), CDK4 (1:1,000, #12790, CST), p27 (1:1,000, #3686, CST) cyclin E1 (1:1,000, #20808, CST), CDK2 (1:1,000, #18048, CST), p21 (1:1,000, #2947, CST, CDK6 (1:1,000, #13331, CST), p53 (1:1,000, #9282, CST), p-Rb (1:1,000, #8516, CST). Each experiment was performed in triplicate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!