The Transwell system (8 μm pore size, Corning, Glendale, AZ, USA) was employed for migration and invasion assays. For the migration assay, 3 x 105 cells were seeded on the upper chambers in serum-free medium. For the invasion assay, 3 x 105 cells were seeded on the upper chamber of a Matrigel-coated Transwell system (8 μm pore size, Corning) in serum-free medium. The bottom chamber was filled with medium supplemented with 5 % fetal bovine serum. After incubation for 24 h at 37 °C, the cells that migrated or invaded through to the bottom of the insert membrane were fixed, stained with crystal violet, and counted under a phase-contrast microscope (Carl Zeiss, biological triplicates).
Matrigel coated transwell system
Manufactured by Corning
The Matrigel-coated Transwell system is a cell culture tool used for studying cell migration and invasion. It consists of a porous membrane insert that is coated with Matrigel, a basement membrane extract. This system provides a controlled environment for cells to migrate through the porous membrane, mimicking the process of cell invasion and metastasis.
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2 protocols using matrigel coated transwell system
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Cell Migration and Invasion Assay
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Cell Migration and Invasion Assay
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Western Blot
Cell migration and invasion assay 4 were gently removed using cotton swabs. The cells that had migrated to the lower surface of the membrane under an inverted light microscope. Cell invasion was assessed using a Matrigel-coated Transwell system (Corning) following procedures as above. Each experiment was conducted in triplicate.
Cell migration and invasion assay 4 were gently removed using cotton swabs. The cells that had migrated to the lower surface of the membrane under an inverted light microscope. Cell invasion was assessed using a Matrigel-coated Transwell system (Corning) following procedures as above. Each experiment was conducted in triplicate.
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