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L malic dehydrogenase

Manufactured by Merck Group

L-malic dehydrogenase is an enzyme that catalyzes the reversible oxidation of L-malate to oxaloacetate, using the coenzyme NAD+ or NADP+. It is an important enzyme in the tricarboxylic acid cycle and plays a role in the interconversion of various organic acids.

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2 protocols using l malic dehydrogenase

1

Enzymatic Assay for Metabolic Intermediates

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ATP, ADP, PEP, OAA, NADH, Glc6P, L-malic acid, pyruvate kinase, and L-malic dehydrogenase were from Sigma Aldrich. L-lactate dehydrogenase was from Roche. All other reagents were of the highest available quality.
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2

Enzyme-Coupled Metabolite Assay

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ATP, ADP, PEP, OAA, NADH, Glc6P, L-malic acid, pyruvate kinase, and L-malic dehydrogenase were from Sigma Aldrich. L-Lactate dehydrogenase was from Roche. All other reagents were of the highest available quality.
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