Mir 21a 5p primer

Manufactured by Takara Bio

Sourced in China

The MiR-21a-5p primer is a DNA oligonucleotide designed for the detection and quantification of the microRNA miR-21a-5p. It is intended for use in molecular biology applications, such as real-time PCR and other nucleic acid analysis techniques.

Automatically generated - may contain errors

Lab products found in correlation

Sybr green premix pro taq hs qpcr kit, by Accurate Biology (2 mentions) Mir xtm mirna first strand synthesis kit, by Takara Bio (2 mentions) Trizol, by Accurate Biology (2 mentions) Exo m mlv rt kit with gdna clean for qpcr 2, by Accurate Biology (2 mentions) Lightcycler 480ii fast real time pcr system, by Roche (2 mentions)

2 protocols using mir 21a 5p primer

Quantifying miRNA Expression in Astrocytes and Spinal Cord

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TRIzol^TM (Accurate Biology, Hunan, China) was used to extract total RNA from astrocytes or spinal cord tissue. The SpectraMax®QuickDrop^TM spectrophotometer was used to detect the concentration of total RNA. Total RNA was stored at -80 °C or immediately used.
The Mir-X^TMmiRNA First-Strand Synthesis Kit (Takara, Dalian, China) and miR-21a-5p primer (Takara) were used to detect the expression level of miR-21a-5p, and U6 was used as an endogenous control to normalize the results. Total RNA (1000 ng) was prepared for reverse transcription using Exo M-MLV RT Kit with gDNA Clean for qPCR II (Accurate Biology). qRT-PCR was performed using the SYBR®Green Premix Pro Taq HS qPCR Kit (Accurate Biology) and a LightCycler®480II Fast Real-Time PCR System (Roche, Switzerland). GAPDH was used as an endogenous control to normalize the results. The results were analyzed using the 2^-ΔΔCT method. The primer pairs used are listed in Table 1.

Zhang Y., Meng T., Chen J., Zhang Y., Kang J., Li X., Yu G., Tian L., Jin Z., Dong H., Zhang X, & Ning B. (2021). miR-21a-5p Promotes Inflammation following Traumatic Spinal Cord Injury through Upregulation of Neurotoxic Reactive Astrocyte (A1) Polarization by Inhibiting the CNTF/STAT3/Nkrf Pathway. International Journal of Biological Sciences, 17(11), 2795-2810.

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Quantification of miR-21a-5p Expression in Astrocytes and Spinal Cord Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

TRIzol TM (Accurate Biology, Hunan, China) was used to extract total RNA from astrocytes or spinal cord tissue. The SpectraMax®QuickDrop TM spectrophotometer was used for detecting the concentration of total RNA. The total RNA was stored at -80℃ or directly used.
Mir-X TM miRNA First-Strand Synthesis Kit (Takara, Dalian, China) and miR-21a-5p primer (Takara) were used to detect the expression level of miR-21a-5p, and U6 was used as an endogenous control to normalize the results. The 1000ng total RNA was prepared for reverse transcription using Exo M-MLV RT Kit with gDNA Clean for qPCR II (Accurate Biology). We performed qRT-PCR with the SYBR®Green Premix Pro Taq HS qPCR Kit (Accurate Biology) at LightCycler®480II Fast Real-Time PCR System (Roche, Switzerland). GAPDH was used as an endogenous control to normalize the results. The results were calculated with the value 2 -ΔΔCT . The primer pairs used are in Table . 1.

Zhang Y., Meng T., Chen J., Zhang Y., Kang J., Li X., Yu G., Tian L., Jin Z., Dong H., & Ning B. (2020). mir-21a-5p promote the progress of inflammation after Traumatic Spinal Cord Injury via up-regulating neurotoxic reactive astrocyte (A1) polarization by inhibiting CNTF/STAT3/Nkrf pathway.

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