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Agar is a gelatinous substance derived from red algae. It is used as a solidifying agent in microbiological culture media, allowing for the growth and isolation of bacteria and other microorganisms.

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Lab products found in correlation

D glucose, by Avantor (1 mentions) Ampicillin, by Merck Group (1 mentions) Growth chamber, by Conviron (1 mentions)

2 protocols using agar

1

Canavanine Resistance Assay Protocol

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Canavanine plates were made by adding 20 g D-glucose (VWR), 6.7 g Difco™ Yeast Nitrogen Base without Amino Acids (Bioworld), 2.0 g Drop-Out Mix Synthetic Minus Arginine without Yeast Nitrogen Base (US Biological), and 20 g agar (Research Products International (RPI); Mt. Prospect, IL) to 1 L of DI water and autoclaving at 120 °C for 15 minutes. When the mixture had cooled to about 55 °C, canavanine sulfate (Sigma; final concentration: 30 mg/L) and ampicillin (Sigma; final concentration: 100 mg/L) were added and the mixture allowed to stir before pouring into 15 x 100 mm plates (about 25 mL per plate). Plates were allowed to set overnight and stored at 4 °C until use.
Ong J.Y., Pence J.T., Molik D.C., Shepherd H.A, & Goodson H.V. (2021). Yeast grown in continuous culture systems can detect mutagens with improved sensitivity relative to the Ames test. PLoS ONE, 16(3), e0235303.
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2

Growth Conditions for Arabidopsis Accessions

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All plants were grown in a Conviron growth chamber under 24 h of light ( 100 µmol m -2 s -1 ) at 22°C. Seeds sown on plates were grown vertically on plant growth media (GM) containing 0.5× MS basal salts, 1% w/v sucrose, 0.05% w/v MES (pH5.8), and 0.8% w/v agar (Research Products International Cas #: 9002-18-0).
Details for accessions used in this study are listed in Supplemental Table S1. bps1-2 (Salk_014042), bps2-2 (CS93151) (which are both Col-0 alleles), and the Apost-1 accession (CS76368), as well as all other accessions, were ordered from the Arabidopsis Biological Resource Center. pCYCB1;1::GUS is described in Colón-Carmona et al. (1999) (link).
Cummins A.J., Siler C.J., Olson J.M., Kaur A., Hamdani A.K., Olson L.K., Dilkes B.P, & Sieburth L.E. (2023). A cryptic natural variant allele of BYPASS2 suppresses the bypass1 mutant phenotype. Plant physiology, 192(2).
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