Captiva filtration equipment

SHetA2 was synthesized by Cayman Chemical company, Inc. (Ann Arbor, MI) under a contract from the Rapid Access to Preventive Intervention Development (RAPID) National Cancer Institute (NCI) program. Cocoa butter was purchased from Nature’s Oils (Streetsboro, OH). Kolliphor HS15 was obtained from BASF (Germany). Sterile saline and isoflurane were obtained from Henry Schein Animal Health Inc. Acetonitrile (HPLC grade ≥ 99.5%), methanol (HPLC grade ≥ 99.5%), phosphoric acid, hydrochloric acid, crystal violet stain and sodium acetate trihydrate were purchased from Sigma Aldrich (St Louis, MO). Captiva^® filtration equipment was purchased from Agilent Technologies Inc. for extraction of drug from tissues. Mouse cyclin D1 enzyme linked immunosorbent assay kit (ELISA) was purchased from Cedarlane lab (NC). T-PER (tissue protein extraction reagent) was purchased from Thermo-Fisher Scientific (Waltham, MA). Protease inhibitor cocktail tablets were purchased from Sigma Aldrich (St Louis, MO).

SHetA2 was synthesized by Cayman Chemical company, Inc. under a contract from the Rapid Access to Preventive Intervention Development (RAPID) National Cancer Institute (NCI) program. Cocoa butter was purchased from Nature’s Oils (Streetsboro, OH). Kolliphor was obtained from BASF (Germany). Sterile saline and isoflurane were obtained from Henry Schein Animal Health Inc. Acetonitrile (HPLC grade ≥ 99.5 %), methanol (HPLC grade ≥ 99.5 %), phosphoric acid, hydrochloric acid, crystal violet stain and sodium acetate trihydrate were purchased from Sigma Aldrich (St Louis, MO). Captiva^® filtration equipment was purchased from Agilent Technologies Inc. for extraction of drug from tissues. Mouse cyclin D1 enzyme linked immunosorbent assay kit (ELISA) was purchased from Cedarlane lab (NC). T-PER (tissue protein extraction reagent) was purchased from Thermo-Fisher Scientific (Waltham, MA). Protease inhibitor cocktail tablets were purchased from Sigma Aldrich (St Louis, MO).