2 3 5 6 tetramethylpyrazine standard

Reagents, strains and media Primers were synthesized by GENEWIZ (Suzhou, China). Plasmids were extracted using the Axyprep TM Plasmid Miniprep Kit (Axygen, USA) and the SanPrep Column Plasmid Mini-Prep Kit (Sangon Biotech, Shanghai, China). BHI Broth was purchased from Hopebio (Qingdao, China). Yeast extract was purchased from Angel (Hubei, China). Acetoin, creatine, 1-naphthol, ethyl 2-acetoxy-2-methylacetoacetate, oxaloacetate and 5,5'dithiobis(2-nitrobenzoic) acid were purchased from Sigma (Merck, USA). The 2,3,5,6-tetramethylpyrazine standard was purchased from Tokyo Chemical Industry (Tokyo, Japan). Other reagents were purchased from Sangon Biotech (Shanghai, China).
The strains and plasmids used in this study were listed in Table 1. Escherichia coli DH5α was used for plasmid construction and was grown in lysogeny broth (LB) medium containing (per liter) 10 g tryptone, 5 g yeast extract, and 10 g NaCl. Brain heart infusion (BHI) medium was used for the transformation and tube culture of C. glutamicum. CGIII medium composed of (per liter) 10 g tryptone, 10 g yeast extract, 2.5 g NaCl and 20 g glucose was used for pre-cultures of C. glutamicum. Batch fermentation of Acetoin was conducted in CGXIIP medium containing (per liter): 10 g yeast extract, 5 g (NH 4 ) 2 SO 4 , 5 g urea, 1 g KH

Reagents, strains and media Primers were synthesized by GENEWIZ (Suzhou, China). Plasmids were extracted using the Axyprep TM Plasmid Miniprep Kit (Axygen, USA) and the SanPrep Column Plasmid Mini-Prep Kit (Sangon Biotech, Shanghai, China). BHI Broth was purchased from Hopebio (Qingdao, China). Yeast extract was purchased from Angel (Hubei, China). Acetoin, creatine, 1-naphthol, ethyl 2-acetoxy-2-methylacetoacetate, oxaloacetate and 5,5'-dithiobis(2-nitrobenzoic) acid were purchased from Sigma (Merck, USA). The 2,3,5,6-tetramethylpyrazine standard was purchased from Tokyo Chemical Industry (Tokyo, Japan). Other reagents were purchased from Sangon Biotech (Shanghai, China).
The strains and plasmids used in this study were listed in Table 1. Escherichia coli DH5α was used for plasmid construction and was grown in lysogeny broth (LB) medium containing (per liter) 10 g tryptone, 5 g yeast extract, and 10 g NaCl. Brain heart infusion (BHI) medium was used for the transformation and tube culture of C. glutamicum. CGIII medium composed of (per liter) 10 g tryptone, 10 g yeast extract, 2.5 g NaCl and 20 g glucose was used for pre-cultures of C. glutamicum. Batch fermentation of Acetoin was conducted in CGXIIP medium containing (per liter): 10 g yeast extract, 5 g (NH 4 ) 2 SO