Protein lysate (PMSF: RIPA=1:100) (Solarbio) was used to lyse the heart tissue from the infarct marginal zone. The extracted proteins were subjected to polyacrylamide gel electrophoresis (GenScript, Nanjing, China) and transferred onto polyvinylidene di uoride membranes (Thermo Fisher Scienti c). The membranes were blocked with QuickBlock Western reagent (Beyotime, Beijing, China) and incubated sequentially with CCR2 overnight at 4°C. Subsequently, the washed membranes were incubated with the corresponding HRP-conjugated secondary antibodies (SA00001-2; Proteintech) for 2 h at 25℃. Lastly, a gel imaging system (GE Healthcare, CHI, USA) was used to quantify the protein bands. The western blots were quanti ed using ImageJ software (NIH, MD, USA).
Protein lysate pmsf ripa 1 100
Manufactured by Solarbio
Protein lysate (PMSF: RIPA=1:100) is a reagent used for the extraction and solubilization of proteins from biological samples. It contains a combination of the cell lysis buffer RIPA and the protease inhibitor phenylmethylsulfonyl fluoride (PMSF) in a 1:100 ratio.
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2 protocols using protein lysate pmsf ripa 1 100
1
Quantitative Western Blotting of CCR2
2
Quantitative Western Blotting of CCR2
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Protein lysate (PMSF: RIPA=1:100) (Solarbio) was used to lyse the heart tissue from the infarct marginal zone. The extracted proteins were subjected to polyacrylamide gel electrophoresis (GenScript, Nanjing, China) and transferred onto polyvinylidene di uoride membranes (Thermo Fisher Scienti c). The membranes were blocked with QuickBlock Western reagent (Beyotime, Beijing, China) and incubated sequentially with CCR2 overnight at 4°C. Subsequently, the washed membranes were incubated with the corresponding HRP-conjugated secondary antibodies (SA00001-2; Proteintech) for 2 h at 25℃. Lastly, a gel imaging system (GE Healthcare, CHI, USA) was used to quantify the protein bands. The western blots were quanti ed using ImageJ software (NIH, MD, USA).
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