Crude methanol extract (4 g) was loaded on to a 600 mm  30 mm silica gel (60-120 mesh) column and eluted successively by passing various solvents (150 mL each) of increasing polarities such as petroleum benzene, chloroform, acetone and methanol. The fraction obtained in methanol (AMF), which was found most effective in scavenging free radicals, was used for further studies. Chemicals 7,12-Dimethylbenz[a]anthracene (DMBA) was purchased from Sigma Chemicals (St. Louis, MO). DMBA was dissolved at a concentration of 100 mg/100 mL acetone. Croton oil was also dissolved in acetone to give a solution of 1% dilution. Nutrient broth was purchased from Hi-media Laboratories, Mumbai, India. Agar agar, L-histidine, biotin, glucose-6-phosphate, dimethyl sulphoxide and NaN 3 were obtained from Sisco Research Laboratories, Mumbai, India. NPDA was purchased from Sigma Chemicals. All other chemicals used were of the analytical grade.
Glucose 6 phosphate (g6p)
Manufactured by Sisco Research Laboratories
Sourced in India
Glucose-6-phosphate is a key metabolic intermediate in the glycolysis pathway. It serves as the primary substrate for the enzyme glucose-6-phosphate dehydrogenase, which catalyzes the first step of the pentose phosphate pathway. Glucose-6-phosphate is an essential component in various biochemical and cell-based assays.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulphoxide, by Sisco Research Laboratories (1 mentions)
Nutrient broth, by HiMedia (1 mentions)
2 4 6 tris 2 pyridyl s triazine, by Merck Group (1 mentions)
γ glutamyl p nitroanilide, by Merck Group (1 mentions)
P nitrophenyl phosphate, by Merck Group (1 mentions)
Nano2, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Sisco Research Laboratories (1 mentions)
2 4 dinitrophenylhydrazine, by Sisco Research Laboratories (1 mentions)
Sodium dodecyl sulfate (sds), by Sisco Research Laboratories (1 mentions)
O phthalaldehyde, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid, by Sisco Research Laboratories (1 mentions)
Pyrogallol, by Sisco Research Laboratories (1 mentions)
Hydrogen peroxide h2o2, by Sisco Research Laboratories (1 mentions)
5 5 dithiobisnitrobenzoic acid, by Sisco Research Laboratories (1 mentions)
Proteinase k, by Sisco Research Laboratories (1 mentions)
Thiobarbituric acid, by HiMedia (1 mentions)
Trichloroacetic acid, by HiMedia (1 mentions)
2 protocols using glucose 6 phosphate (g6p)
1
Fractionation and Evaluation of Methanol Extract
2
Biochemical Assays Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Agarose, 1-chloro-2,4-dinitrobenzene, 2,2-diphenyl-1-picrylhydrazyl (DPPH), N-ethylmaleimide, glutathione reductase (GR), L-leucine p-nitroanilide, metaphosphoric acid, NaNO2, Roswell Park Memorial Institute 1640 (RPMI) medium, Triton X-100, 2,4,6-tris(2-pyridyl)-s-triazine, p-nitrophenyl phosphate, γ-glutamyl p-nitroanilide, o-phthalaldehyde and NaNO2 were from Sigma Aldrich, USA. Bovine serum albumin, diphenylamine, 5,5'-dithiobisnitrobenzoic acid (DTNB), ethylenediaminetetraacetic acid (EDTA), glucose 6-phosphate, proteinase K, reduced (GSH) and oxidized (GSSG) glutathione, hydrogen peroxide (H2O2), reduced and oxidized nicotinamide adenine dinucleotide phosphate (NADPH and NADP+), sodium dodecyl sulfate (SDS), sorbitol, tris(hydroxymethyl)aminomethane (Tris), pyrogallol, 2,4-dinitrophenylhydrazine and xylenol orange were from Sisco Research Laboratories, India. Thiobarbituric acid and trichloroacetic acid were from Himedia Laboratories (Mumbai, India).
All other chemicals used were of analytical grade.
All other chemicals used were of analytical grade.
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