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Z648043

Manufactured by Merck Group
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Z648043 is a specialized lab equipment used for scientific research and analysis. It is designed to perform precise measurements and data collection in a controlled laboratory environment. The core function of this product is to provide accurate and reliable data to support various scientific investigations and experiments. No further details on the intended use or specific applications of this equipment can be provided in an unbiased and factual manner.

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Lab products found in correlation

Phelper, by Cell Biolabs (2 mentions) Pei max 40k, by Cell Biolabs (2 mentions) E8263 25ku, by Merck Group (2 mentions) Pei max 40k, by Polysciences (2 mentions)

2 protocols using z648043

1

Enhancer AAV Vector Production

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Enhancer AAV plasmids were maxi-prepped and transfected with PEI Max 40K (Polysciences Inc., catalog # 24765-1) into one 15 cm plate of AAV-293 cells (Cell Biolabs catalog # AAV-100), along with helper plasmid pHelper (Cell BioLabs) and PHP.eB rep/cap packaging plasmid (Chan et al., 2017 (link)), with a total mass of 150 μg PEI Max 40K, 30 μg pHelper, 15 μg rep/cap plasmid, and 15 μg enhancer-AAV vector. The next day medium was changed to 1% FBS, and then after 5 days cells and supernatant were harvested and AAV particles released by three freeze-thaw cycles. Lysate was then treated with benzonase to degrade free DNA (2 μL benzonase, 30 min at 37°C, MilliporeSigma catalog # E8263-25KU), and then cell debris was cleared with low-speed spin (1500 g 10 min). The supernatant containing virus was concentrated over a 100 kDa molecular weight cutoff Centricon column (MilliporeSigma catalog # Z648043) to a final volume of ~150 μL. For highly purified large-scale preps this protocol was altered so that ten plates were transfected and harvested together at 3 days after transfection, and then the crude virus was purified by iodixanol gradient centrifugation.
Mich J.K., Graybuck L.T., Hess E.E., Mahoney J.T., Kojima Y., Ding Y., Somasundaram S., Miller J.A., Kalmbach B.E., Radaelli C., Gore B.B., Weed N., Omstead V., Bishaw Y., Shapovalova N.V., Martinez R.A., Fong O., Yao S., Mortrud M., Chong P., Loftus L., Bertagnolli D., Goldy J., Casper T., Dee N., Opitz-Araya X., Cetin A., Smith K.A., Gwinn R.P., Cobbs C., Ko A.L., Ojemann J.G., Keene C.D., Silbergeld D.L., Sunkin S.M., Gradinaru V., Horwitz G.D., Zeng H., Tasic B., Lein E.S., Ting J.T, & Levi B.P. (2021). Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex. Cell reports, 34(13), 108754.
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2

Enhancer AAV Vector Production

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Enhancer AAV plasmids were maxi-prepped and transfected with PEI Max 40K (Polysciences Inc., catalog # 24765-1) into one 15 cm plate of AAV-293 cells (Cell Biolabs catalog # AAV-100), along with helper plasmid pHelper (Cell BioLabs) and PHP.eB rep/cap packaging plasmid (Chan et al., 2017 (link)), with a total mass of 150 μg PEI Max 40K, 30 μg pHelper, 15 μg rep/cap plasmid, and 15 μg enhancer-AAV vector. The next day medium was changed to 1% FBS, and then after 5 days cells and supernatant were harvested and AAV particles released by three freeze-thaw cycles. Lysate was then treated with benzonase to degrade free DNA (2 μL benzonase, 30 min at 37°C, MilliporeSigma catalog # E8263-25KU), and then cell debris was cleared with low-speed spin (1500 g 10 min). The supernatant containing virus was concentrated over a 100 kDa molecular weight cutoff Centricon column (MilliporeSigma catalog # Z648043) to a final volume of ~150 μL. For highly purified large-scale preps this protocol was altered so that ten plates were transfected and harvested together at 3 days after transfection, and then the crude virus was purified by iodixanol gradient centrifugation.
Mich J.K., Graybuck L.T., Hess E.E., Mahoney J.T., Kojima Y., Ding Y., Somasundaram S., Miller J.A., Kalmbach B.E., Radaelli C., Gore B.B., Weed N., Omstead V., Bishaw Y., Shapovalova N.V., Martinez R.A., Fong O., Yao S., Mortrud M., Chong P., Loftus L., Bertagnolli D., Goldy J., Casper T., Dee N., Opitz-Araya X., Cetin A., Smith K.A., Gwinn R.P., Cobbs C., Ko A.L., Ojemann J.G., Keene C.D., Silbergeld D.L., Sunkin S.M., Gradinaru V., Horwitz G.D., Zeng H., Tasic B., Lein E.S., Ting J.T, & Levi B.P. (2021). Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex. Cell reports, 34(13), 108754.
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