The microdevice channels were designed (see Fig. 1 ) using AutoCAD (Autodesk, San Rafael, CA) and formed into 4.0 cm × 9.0 cm × 0.3 cm PMMA blocks using a CO2 laser cutter (VersaLaser VLS3.50, Universal Laser Systems, Scottsdale, AZ) with the following settings: 70% laser power, 10% cutting speed and 1000 pulses per inch. The microchannel was 0.2 mm wide at the top and 1.6 mm deep. For channel enclosure, the PMMA was heated at 100 °C for 2 min on a benchtop laboratory press (Model 4120, Carver, Wabash, IN), after which an optical adhesive film (4360954, Applied Biosystems) was laminated onto the channel side of the PMMA at 2 bar for 2 min. The microdevice was left to cool to ambient temperature over ~6 min to avoid thermally induced stress. Total cost per device was ~1 US dollar, with the optical adhesive film comprising most of the price; laser cutters can be obtained for a few thousand US dollars. Microfluidic connectors were then attached to the inlet and outlets of the PMMA— PSA microdevice using Solarez UV resin (Vista, CA) and cured under UV light for 2 min.
Versalaser vls3
Manufactured by Universal Systems
The VersaLaser VLS3.50 is a laser engraving and cutting system designed for precise processing of a variety of materials. It features a work area of 24 x 12 inches and can accommodate materials up to 4 inches in thickness. The system utilizes a CO2 laser with adjustable power output to facilitate diverse applications.
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Lab products found in correlation
2 protocols using versalaser vls3
1
Fabrication of PMMA Microfluidic Devices
2
Ionic Gel Conductor Fabrication and Characterization
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Example 1
The ionic gel conductors were prepared by dissolving Acrylamide (AAm monomer) powder and NaCl into the deionized water. Molar concentration of AAm was fixed as 2.2 M throughout the entire experiments, and molar concentration of NaCl was varied from 1.37 to 5.48 M. N,Nmethylenebisacrylamide (MBAA) 0.06 wt.-% and Ammonium persulfate (AP) 0.17 wt.-% with respect to the weight of AAm monomer were added as a cross-linker for AAm and a photo initiator, respectively. After degassing in vacuum chamber, N,N,N′,N′-tetramethylethylenediamine (TEMED) 0.25 wt.-% with respect to the weight of AAm monomer were lastly added as the accelerator. The solutions were poured into a glass mold which has 100.0×100.0×0.1 mm3 size vacancy and covered with 3 mm thick transparent glass plate. The gels were cured by the ultraviolet light cross-linker (UVC 500, Hoefer) for 20 min with 8 W power and 254 nm wavelength. The gels were then cut into the designed shape by using laser cutting system (VersaLaser VLS3.50, Universal Laser Systems) with 50 W power and 14 cm/sec beam speed. Before stacking hydrogel on top of VHB, the surfaces of the hydrogels were dried with N2 gas for 1 minute to improve the adhesion between gel and VHB by removing water from the gel surfaces.
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