Peg maleimide

Manufactured by NOF corporation

Sourced in Japan, United States

PEG-maleimide is a chemical compound used in various laboratory applications. It is a polyethylene glycol (PEG) derivative with a maleimide functional group. PEG-maleimide is commonly used for protein conjugation and modification in biochemical and biotechnological research.

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4 protocols using peg maleimide

PEGylation of Recombinant Protein

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To couple PEG to the C-modified N-terminus of pMOG, 157 mg of 20 kDa PEG-maleimide (NOF Corporation, Japan) and 20 mg pMOG were reacted in 20 mL of a 20 mmol/l sodium phosphate buffer pH 7.2 containing 250 µmol/l of Tris(2-carboxyethyl)phosphine. The reaction mixture was stirred at RT for 1 h. Progress of the reaction was monitored by RP-HPLC.

Pfeil J., Simonetti M., Lauer U., Thülen B.v., Durek P., Poulsen C.G., Pawłowska J., Kröger M., Krähmer R., Leenders F., Hoffmann U., & Hamann A. (2020). Prevention of EAE by PEGylated Antigenic Peptides.

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PEGylation of pMOG Protein

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To couple PEG to the C-modified N-terminus of pMOG, 157 mg of 20 kDa PEG-maleimide (NOF Corporation, Japan) and 20 mg pMOG were reacted in 20 mL of a 20 mmol/L of sodium phosphate buffer pH 7.2 containing 250 µmol/L of Tris(2-carboxyethyl)phosphine. The reaction mixture was stirred at RT for 1 h. Progress of the reaction was monitored by RP-HPLC.

Pfeil J., Simonetti M., Lauer U., Volkmer R., von Thülen B., Durek P., Krähmer R., Leenders F., Hamann A, & Hoffmann U. (2020). Tolerogenic Immunomodulation by PEGylated Antigenic Peptides. Frontiers in Immunology, 11, 529035.

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PEGylation of Engineered Protein

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To couple PEG to the C-modified N-terminus of pMOG, 157 mg of 20 kDa PEG-maleimide (NOF Corporation, Japan) and 20 mg pMOG were reacted in 20 mL of a 20 mmol/l sodium phosphate buffer pH 7.2 containing 250 µmol/l of Tris(2-carboxyethyl)phosphine. The reaction mixture was stirred at room temperature (RT) for 1 h. Progress of the reaction was monitored by reversed-phase high-performance liquid chromatography (RP-HPLC).

Pfeil J., Simonetti M., Lauer U., von Thülen B., Durek P., Poulsen C., Pawlowska J., Kröger M., Krähmer R., Leenders F., Hoffmann U, & Hamann A. (2021). Prevention of EAE by tolerogenic vaccination with PEGylated antigenic peptides. Therapeutic Advances in Chronic Disease, 12, 20406223211037830.

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PEGylation of OVA323-339 Peptide

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A 12-kDa PEG-maleimide was obtained from Quanta Biodesign (USA), 20 and 40 kDa PEG-maleimide from NOF Corporation (Japan). To couple PEG to the CG-modified N-terminus of pOVA_323–339, 450 mg of 20 kDa PEG-maleimide or 900 mg of 40 kDa PEG-maleimide and 40 mg CG-pOVA_323–339 were reacted in 40 mL of a 50 mmol/L of sodium phosphate buffer (pH 7.0). The reaction mixture was stirred at RT for 1 h. Progress of the reaction was monitored by RP-HPLC.

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