HRCPs (Cambrex, Walkersville, MD, USA) were cultured in EBM-2 medium. Cells (passages 3–9) at 85% confluence were treated with either N-LDL or HOG-LDL as indicated. Where appropriate, cells were pre-treated with pharmacological reagents prior to lipoprotein exposure. See ESM Methods for details.
Hrcps
Manufactured by Cambrex
Sourced in United States
HRCPs are laboratory equipment used for the purification and separation of various biomolecules, such as proteins, enzymes, and nucleic acids. They utilize high-resolution chromatographic techniques to efficiently isolate and concentrate target compounds from complex mixtures.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using hrcps
1
Endothelial Cell Lipoprotein Treatment
2
Glucose and Mannose Effects on HRCP
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HRCPs (Cambrex Biosciences, Walkersville, MD, USA) were cultured in DMEM (Solarbio, Beijing, China) at 37°C and 5% CO2. DMEM contained 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Solarbio). HRCPs were incubated with 5 mM glucose (normal glucose, NG), 30 mM glucose (HG) or 5 mM glucose + 25 mM mannose (Man) with or without 10 mM DAC for 4 d.
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