Yeast strain, the vector, and antibiotics were supplied from Invitrogen (USA). Phytic acid and sodium salt were purchased from Sigma-Aldrich (USA). Sodium acetate, sodium carbonate, sodium thiosulphate, ammonium heptamolybdate tetrahydrate, sulfuric acid 95–98%, glacial acetic acid, acetone, and glycine were supplied from Merck-Millipore (Germany). The plasmid DNA extraction kit, yeast genomic DNA extraction kit, and RNA extraction kit were obtained from SinaClon (Iran). Restriction enzymes were purchased from Thermo Scientific (USA). Commercial phytase was from Novozyme (Denmark).
Rna extraction kit
Manufactured by Sinaclon
Sourced in United States
The RNA extraction kit is a laboratory equipment designed to isolate and purify RNA molecules from various biological samples. It provides a standardized and efficient method for extracting high-quality RNA for downstream applications such as gene expression analysis, RT-PCR, and RNA sequencing.
Automatically generated - may contain errors
Lab products found in correlation
Nanodrop, by Thermo Fisher Scientific (2 mentions)
Phytic acid, by Merck Group (1 mentions)
Ammonium heptamolybdate tetrahydrate, by Merck Group (1 mentions)
Sodium thiosulphate, by Merck Group (1 mentions)
Glycine, by Merck Group (1 mentions)
Restriction enzyme, by Thermo Fisher Scientific (1 mentions)
Acetone, by Merck Group (1 mentions)
Sodium acetate, by Merck Group (1 mentions)
Sodium carbonate, by Merck Group (1 mentions)
Alloxan monohydrate, by Merck Group (1 mentions)
Rat reactive oxygen species elisa kit, by MyBioSource (1 mentions)
Triglyceride quantification assay kit, by Abcam (1 mentions)
Primescript rt reagent kit, by Takara Bio (1 mentions)
2019 ncov nucleic acid diagnostic kit pcr fluorescence probing, by Sansure Biotech (1 mentions)
3 protocols using rna extraction kit
1
Yeast-based Phytic Acid Quantification
2
Rat Model of Oxidative Stress and Lipid Metabolism
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In this experimental study, chemicals and reagents were
obtained from the following sources: Alloxan monohydrate
(Sigma, St. Louis, MO, USA), RNA Extraction kit
(SinaClon Co., Iran), PrimeScript RT Reagent kit
(TaKaRa Biotechnology, Japan), miRNA isolation kit and
Super SYBR Green qPCR Master Mix 2x (Yekta Tajhiz
Co., Iran), Universal cDNA synthesis kit II and ExiLENT
SYBR® Green PCR Master Mix (Exiqon, USA), Rat
Reactive Oxygen Species ELISA kit (MyBioSource, San
Diego, CA, USA), Triglyceride Quantification Assay kit
(Abcam, USA), and commercial Free Fatty Acid Assay
kit (Enzychrom Bio-Assays Systems, USA). All other
chemicals and solvents were of the highest commercial
grade and purchased from either Merck (KGaA,
Germany) or Sigma (St. Louis, MO, USA). We purchased
male Wistar rats (Rattus norvegicus) that weighed 180-
220 g from the School of Pharmacy, Tehran University
of Medical Sciences (Iran). Animals were housed 4 per
standard rat cage in a room with a 12:12 hour light/dark
cycle and controlled temperature (24 ± 1˚C). The animals
were allowed to adapt to their new location for one week.
Animals received water and a standard diet (27% protein,
32% fat, and 41% carbohydrate) ad libitum.
obtained from the following sources: Alloxan monohydrate
(Sigma, St. Louis, MO, USA), RNA Extraction kit
(SinaClon Co., Iran), PrimeScript RT Reagent kit
(TaKaRa Biotechnology, Japan), miRNA isolation kit and
Super SYBR Green qPCR Master Mix 2x (Yekta Tajhiz
Co., Iran), Universal cDNA synthesis kit II and ExiLENT
SYBR® Green PCR Master Mix (Exiqon, USA), Rat
Reactive Oxygen Species ELISA kit (MyBioSource, San
Diego, CA, USA), Triglyceride Quantification Assay kit
(Abcam, USA), and commercial Free Fatty Acid Assay
kit (Enzychrom Bio-Assays Systems, USA). All other
chemicals and solvents were of the highest commercial
grade and purchased from either Merck (KGaA,
Germany) or Sigma (St. Louis, MO, USA). We purchased
male Wistar rats (Rattus norvegicus) that weighed 180-
220 g from the School of Pharmacy, Tehran University
of Medical Sciences (Iran). Animals were housed 4 per
standard rat cage in a room with a 12:12 hour light/dark
cycle and controlled temperature (24 ± 1˚C). The animals
were allowed to adapt to their new location for one week.
Animals received water and a standard diet (27% protein,
32% fat, and 41% carbohydrate) ad libitum.
3
SARS-CoV-2 Detection via One-Step RT-PCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The collected air samples were transferred to a virology lab in cold chain and were kept at −70 °C. Total RNAs were extracted using an RNA Extraction Kit (Sinaclon Co, Tehran, IRAN). The purity of extracted RNAs was determined by Nanodrop (ThermoScientific, USA). One-step reverse transcriptase real-time PCR was performed for RNA-dependent RNA polymerase (RdRp) and N SARS-CoV-2 genes with specific primers and probes (FAM and Texas red) with the use of a One Step Novel Coronavirus (2019-NCOV) Nucleic Acid Diagnostic Kit (PCR-Fluorescence Probing, SANSURE Biotech, china). The internal control primer and probe (CY5) were evaluated. Thermal cycling conditions were as follows: 50 °C for 30 min, 95 °C for 1 min, followed by 45 cycles of 95 °C for 15 s, 60 °C for 30 s. The RT-PCR cycle threshold (CT) values ≤ 40 and a sigmoidal curve were considered as positive results for both genes. The sampling and extraction were repeated for samples in which the Ct of the Internal Control gene was not determined.
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