All of the amino acids (alanine, arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, proline, serine, tyrosine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, ornithine, and citrulline), pyroglutamic acid, urocanic acid, urea, and lactic acid were purchased from Wako (Osaka, Japan). All of the SIL-IS were purchased from JUNSEI (Tokyo, Japan). Deionized water was produced in-house using a Milli-Q gradient water purification system (Merck Millipore, Darmstadt, Germany). The SIL-IS mixture solution containing all of the SIL compounds was prepared in deionized water with each having a final concentration of 0.5 μmol/mL. The NMF mixture solutions containing all of the amino acids and pyroglutamic acid, urocanic acid, urea, lactic acid were prepared in deionized water with a final concentration ranging between 0.025–12.5 μmol/mL for each compound.
Lysine
Manufactured by Fujifilm
Sourced in Japan
Lysine is a laboratory equipment product manufactured by Fujifilm. It is an essential amino acid that plays a crucial role in various biological processes. The core function of Lysine is to support protein synthesis and metabolism within cells.
Automatically generated - may contain errors
Lab products found in correlation
Histidine, by Fujifilm (4 mentions)
Leucine, by Fujifilm (4 mentions)
Glycine, by Fujifilm (3 mentions)
Cysteine, by Fujifilm (3 mentions)
Alanine, by Fujifilm (3 mentions)
Asparagine, by Fujifilm (3 mentions)
Aspartic acid, by Fujifilm (3 mentions)
Glutamic acid, by Fujifilm (3 mentions)
Serine, by Fujifilm (3 mentions)
Tyrosine, by Fujifilm (3 mentions)
Isoleucine, by Fujifilm (3 mentions)
Methionine, by Fujifilm (3 mentions)
Phenylalanine, by Fujifilm (3 mentions)
Threonine, by Fujifilm (3 mentions)
Valine, by Fujifilm (3 mentions)
Glutamine, by Fujifilm (2 mentions)
Proline, by Fujifilm (2 mentions)
Arginine, by Fujifilm (2 mentions)
Tryptophan, by Fujifilm (2 mentions)
Urea, by Fujifilm (1 mentions)
4 protocols using lysine
1
Amino Acid Quantification Protocol
2
Amino Acid Characterization Protocol
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NX, Glycine, alanine, valine, leucine, isoleucine, methionine, serine, threonine, cysteine, asparagine, phenylalanine, tyrosine, tryptophan, lysine, histidine, aspartic acid, glutamic acid, methanol, ethanol, hydrochloric acid (HCl), and acetic acid were purchased from Fujifilm Wako Pure Chemical Corporation (Osaka, Japan). Proline, glutamine, and arginine were purchased from the Tokyo Chemical Industry Corporation (Tokyo, Japan). All amino acids used in this experiment were of DL-form. Milli-Q water (18.2 mΩ/cm) was prepared by using a Milli-Q water purification system (Merck, Darmstadt, Germany).
3
Fungal Growth Media for Fission Yeast
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YES medium contains 0.5% yeast extract (Gibco), 3% glucose (FUJIFILM Wako), and 0.1 mg/ml each of adenine (Sigma-Aldrich), uracil (Sigma-Aldrich), leucine (FUJIFILM Wako), lysine (FUJIFILM Wako), and histidine (FUJIFILM Wako). YES plates were made by adding 2% agar (Gibco) to the YES medium. Synthetic dextrose minimal medium (SD) contains 6.3 g/liter Yeast Nitrogen Base w/o Amino Acids (BD), 2% glucose, and 0.1 mg/ml each of the required amino acids. Edinburgh Minimal Medium (EMM) contains 12.3 g/liter EMM Broth without Nitrogen (Formedium), 2% glucose, and 0.1 mg/ml each of required amino acids. Pombe Minimal Glutamate (PMG) contains 27.3 g/liter EMM Broth without Nitrogen, 5 g/liter L-glutamic acid (Sigma-Aldrich), and 0.1 mg/ml each of required amino acids. 15 µM thiamine (Sigma-Aldrich) was added to EMM or PMG medium to repress the nmt1 promoter activity. Yeast strains and plasmids used in this study are listed in Tables 1 and 2 .
4
Spectroscopic Analysis of Amino Acid Interactions
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Asparagine, glycine, alanine, arginine, aspartic acid, cysteine, glutamine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, valine, Trp, 10% hydrochloric acid (HCl), sodium hydroxide (NaOH), ascorbic acid (AA), sodium chloride (NaCl), D (+)-glucose, lysozyme, and glyoxylic acid monohydrate were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). MSG monohydrate and NaOCl·5H2O were purchased from Nacalai Tesque (Kyoto, Japan). Pooled Human Cerebrospinal Fluid (CSF) was purchased from Lee Biosolutions, Inc. (USA, Missouri).
A block incubator, WSC-2620 PowerBLOCK (ATTO Corporation, Tokyo, Japan), was used to set and maintain the temperature (10, 20, 30, 40, 50, 60, and 70°C) of sample solutions.
A double-beam spectrophotometer U-2900 (Hitachi High-Technology Co., Ltd., Tokyo, Japan) with microcells and a 10-mm path length was used to measure the absorption spectra of sample solutions.
A block incubator, WSC-2620 PowerBLOCK (ATTO Corporation, Tokyo, Japan), was used to set and maintain the temperature (10, 20, 30, 40, 50, 60, and 70°C) of sample solutions.
A double-beam spectrophotometer U-2900 (Hitachi High-Technology Co., Ltd., Tokyo, Japan) with microcells and a 10-mm path length was used to measure the absorption spectra of sample solutions.
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