The H9c2 rat cardiomyoblast cell line was purchased from ATCC (n° CRL-1446, Manassas, VA, USA). Cells were cultured in DMEM medium with 1 mM pyruvate and 4.5 g/L glucose (Fisher Scientific, Illkirch, France) and supplemented with 100 U/mL penicillin, 100 mg/mL streptomycin (Fisher Scientific, Illkirch, France), and 10% FBS (Corning, Glendale, AZ, USA) at 37 °C under 5% CO2/95% air. Severe ER stress was induced by TN treatment at 10 µg/mL (Tocris, Bristol, UK). EX-527 Sirtuin-1 inhibitor was used at 5 µM (Tocris, Bristol, UK). Its IC50 value was determined using the in vitro Fluor de Lys deacetylation assay and a purified human SIRT1 range from 38 to 150 nM. Berberine, Catechin, Ferulic Acid, Malvidin, Tyrosol, and Resveratrol were purchased from Merck-Sigma (Saint-Quentin-Fallavier, France). Butein, Isoliquiritigenin, Piceatannol, and Pterostilbene were purchased from Cayman (Ann Arbor, MI, USA). All phenolic phytochemicals were diluted in DMSO.
Pterostilbene
Manufactured by Cayman Chemical
Sourced in United States
Pterostilbene is a laboratory standard chemical produced by Cayman Chemical. It is a dimethylated analog of the stilbenoid resveratrol. Pterostilbene serves as a reference standard for analytical and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Malvidin, by Merck Group (1 mentions)
Pyruvate, by Thermo Fisher Scientific (1 mentions)
Tyrosol, by Merck Group (1 mentions)
Berberine, by Merck Group (1 mentions)
Glucose, by Thermo Fisher Scientific (1 mentions)
H9c2 rat cardiomyoblast cell line, by American Type Culture Collection (1 mentions)
Piceatannol, by Cayman Chemical (1 mentions)
Butein, by Cayman Chemical (1 mentions)
Isoliquiritigenin, by Cayman Chemical (1 mentions)
Resveratrol, by Merck Group (1 mentions)
Catechin, by Merck Group (1 mentions)
Ferulic acid, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Resveratrol, by Cayman Chemical (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Dmem f12 1 1 medium, by Thermo Fisher Scientific (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
4 protocols using pterostilbene
1
Comparative Analysis of Phytochemical Effects on ER Stress
2
Assessing Antifungal Effects of Stilbenoids
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Initial testing was done using L. maculans isolate D6, which is virulent towards the Rlm3, Rlm4 and Rlm9 major genes found in Australian cultivars [35 (link)]. Hyphal plugs (4 mm diameter) were excised from the edge of 13 day old cultures and transferred into the centre of 36 mm petri dishes containing 3 ml of half strength V8 agar amended either with resveratrol (Cayman Chemical, USA) or pterostilbene (Cayman Chemical, USA) from 50 mg/ml stock solutions in 100% EtOH to final concentrations of 25, 50, 100, 200 and 400 μg/ml. Control (solvent only) cultures had identical ethanol concentrations as treatments. Cultures were incubated at 18°C under the conditions described previously. There were three replicate culture plates of each treatment and the experiment was repeated once. Mycelial growth diameters (mm) were measured in two directions at right angle and recorded daily. After six days, mycelial growth was expressed as the percentage (%) of growth in the treatment relative to the control (100%) and the effective concentration (EC50) in which mycelial growth was reduced by 50% was calculated using GraphPad Prism® software version 6.07.
3
Comparative Cytotoxicity of Chemotherapeutics in Bladder Cancer
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The chemicals used in this study were obtained from Sigma (St. Louis, MO, USA) unless otherwise indicated. Pterostilbene (>98% purity) was obtained from Cayman Chemical (Ann Arbor, MI, USA). Cisplatin (Abiplatin®, 0.5 g/mL) was obtained from Abic Ltd. (Netanya, Israel). Carboplatin (Paraplatin®, 10 mg/mL) was obtained from Corden Pharma Latina S. P. A. (Sermoneta, LT, Italy). Gemcitabine (Gemzar®) was obtained from Lilly USA, LLC (Indianapolis, IN, USA). Pterostilbene was dissolved in DMSO. Gemcitabine was dissolved in water. Grade III human bladder cancer T24 cells (ATCC, Rockville, MD, USA) and immortalized human uroepithelial E7 cells (a gift from Dr. Nan-Haw Chow; National Cheng Kung University Hospital, Tainan, Taiwan) [71 (link)] were cultured in complete Dulbecco’s modified Eagle medium (DMEM; GIBCO BRL, Gaithersburg, MD, USA) in an incubator at 37 °C with a humidified atmosphere of 5% CO2. All of the compounds were diluted to the final desired concentrations with complete DMEM. Control cells were treated with an equal amount of DMSO (0.1%) without Pterostilbene, cisplatin, carboplatin, or gemcitabine.
4
Resveratrol and Pterostilbene in Breast Cancer
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Human mammary epithelial MCF10A cell line and human breast cancer MCF10CA1h and MCF10CA1a cell lines were cultured in DMEM/F12 (1:1) medium (Gibco) supplemented with 5% horse serum (Gibco), 1U/ml penicillin and 1µg/ml streptomycin (Gibco). Medium for Louis, MO, USA), and 500 ng/ml hydrocortisone (Sigma-Aldrich, St. Louis, MO, USA).
MCF10CA1h and MCF10CA1a breast cancer cells used in our experiments were derived from tumor xenografts of MCF10A cells transformed with constitutively active Harvey-ras oncogene, and represent respectively well-and poorly-differentiated malignant tumors. All cell lines were routinely verified by morphology, invasion and growth rate. Cell lines were authenticated by DNA profiling using the short tandem repeat (ATCC). Cells, grown in a humidified atmosphere of 5% carbon dioxide at 37°C, were treated with resveratrol (RSV, Sigma-Aldrich, St. Louis, MO, USA) or pterostilbene (PTS, Cayman Chem., Ann Arbor, MI, USA) freshly resuspended in ethanol. 24 h prior to treatments, cells were plated at a density of 2-3 x 10 5 followed by exposure to RSV or PTS at 0-20 µM concentrations for 4 days. Cells were then passaged 1:50 and exposed for additional 4 days (9-day exposure).
MCF10CA1h and MCF10CA1a breast cancer cells used in our experiments were derived from tumor xenografts of MCF10A cells transformed with constitutively active Harvey-ras oncogene, and represent respectively well-and poorly-differentiated malignant tumors. All cell lines were routinely verified by morphology, invasion and growth rate. Cell lines were authenticated by DNA profiling using the short tandem repeat (ATCC). Cells, grown in a humidified atmosphere of 5% carbon dioxide at 37°C, were treated with resveratrol (RSV, Sigma-Aldrich, St. Louis, MO, USA) or pterostilbene (PTS, Cayman Chem., Ann Arbor, MI, USA) freshly resuspended in ethanol. 24 h prior to treatments, cells were plated at a density of 2-3 x 10 5 followed by exposure to RSV or PTS at 0-20 µM concentrations for 4 days. Cells were then passaged 1:50 and exposed for additional 4 days (9-day exposure).
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