Chemically fixed scallop eyes were washed with 0.1 M cacodylate buffer for 2 h and post-fixed with 1% osmium tetroxide and 2% uranyl acetate according to the method published in ref. 17 . Ultra-thin sections were prepared with an ultra-microtome (RMC, Arizona, USA) and imaged with HRSEM Gemini 300 SEM (Zeiss) STEM detector, and Thermo Fisher Scientific (former FEI) Talos F200C transmission electron microscope operating at 200 kV. The images were taken with Ceta 16 M CMOS camera. The electron diffraction patterns were obtained with a Thermo Fisher Scientific (FEI) Tecnai T12 G2 TWIN TEM operating at 120 kV. Images and electron diffraction (ED) patterns were recorded using a Gatan 794 MultiScan CCD camera. ED analysis was done using Gatan Digital Micrograph software with the DIFPack module. Images and ED patterns were recorded with consideration of potential beam damage to the sample, thus appropriate illumination conditions (spot size) were used to avoid it.
794 multiscan ccd camera
Manufactured by Ametek
The 794 MultiScan CCD camera is a high-performance imaging device designed for scientific and industrial applications. It features a charge-coupled device (CCD) sensor that captures images with high resolution and sensitivity. The camera can be used for a variety of imaging tasks, providing reliable and accurate data acquisition.
Automatically generated - may contain errors
Lab products found in correlation
Tecnai t12 g2 twin tem, by Ametek (2 mentions)
Talos f200c, by Thermo Fisher Scientific (1 mentions)
Hrsem gemini 300 sem, by Zeiss (1 mentions)
Tecnai t12 g2 twin tem, by Thermo Fisher Scientific (1 mentions)
Axs d8 advance, by Bruker (1 mentions)
Spectrum two, by PerkinElmer (1 mentions)
Digital micrograph software, by Ametek (1 mentions)
Ultracut uct, by Leica camera (1 mentions)
Jem 1230, by JEOL (1 mentions)
Cm100 microscope, by Ametek (1 mentions)
Tecnai t12 electron microscope, by Thermo Fisher Scientific (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
9 protocols using 794 multiscan ccd camera
1
Ultrastructural Analysis of Scallop Eyes
2
Structural Analysis of Guanine Crystals
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Crystals were isolated from fixed lizard tail tissues and cleaned in purified water, and a drop of the resulting suspension was placed on a glow-discharged Cu meshed TEM grid and allowed to dry. The resulting samples were observed with a FEI Tecnai T12 G2 TWIN TEM operating at 120 kV. Images and electron diffraction patterns were recorded using a Gatan 794 MultiScan CCD camera. The electron diffraction on the sample allows us to determine that the structure of reflecting plates is β-guanine and that individual guanine plates are indeed single crystals.
3
Comprehensive Materials Characterization Protocol
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Morphological analysis was carried out by SEM using a ZEISS 1540XB FE SEM instrument (Carl-Zeiss, Jena, Germany) equipped with an EDX detector. Transmission electron microscopy (TEM) was carried out with a JEOL JEM 2010 electron microscope (LaB6 electron gun) operating at 200 kV, equipped by a Gatan 794 Multi-Scan CCD camera for digital imaging. Samples for TEM analysis were prepared by dropping a suspension of the starting powder, dispersed in isopropanol and sonicated, on a 400 mesh holey-carbon coated copper grid. An X-ray diffractometry (XRD) analysis was carried out on a Bruker AXS D8 Advance diffractometer within the 2θ range of 20 to 80° using CuKα as an X-ray source (λ = 1.5406 Å). ATR-FTIR analyses were performed using a Spectrum Two (Perkin-Elmer) FT-IR spectrometer with a diamond ATR (attenuated total reflection) single reflection accessory.
4
Isolation and Characterization of Nanospheres from Cleaner Shrimp
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The nanospheres were extracted from the white maxillipeds and antennae of a cleaner shrimp (Supplementary Fig. 15 ). To do this, the maxillipeds and antennae were sliced into ~1–2-mm pieces, placed in a glass vial with a few drops of hexane, and sonicated for 30 s in a sonication bath, then 3 µl of the resulting suspension was dropped on a carbon-coated Cu-meshed TEM grid and allowed to dry. The resulting samples were observed with a ThermoFisher Scientific (FEI) Tecnai T12 G2 TWIN TEM operating at 120 kV. Images and electron-diffraction patterns were recorded using a Gatan 794 MultiScan CCD camera. Electron-diffraction analysis was done using Gatan DigitalMicrograph software with the DIFPack module. Particle diameters were measured using Gatan DigitalMicrograph software, and 146 particles were measured to find the average size and standard deviation (Supplementary Table 1 and Supplementary Figs. 16 and 17 ).
5
Characterization of Silver Nanoparticles by TEM and HRTEM
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TEM and HRTEM were performed at an accelerating voltage of 200 kV on a JEOL 3010, 300 kV instrument equipped with a UHR polepiece. A Gatan 794 multiscan CCD camera was used for image acquisition. Energy-dispersive X-ray spectroscopy (EDS) spectra were collected on an Oxford Semistem system housed on the TEM. The formation of NPs during microdroplet deposition was examined directly using 300-mesh carbon-coated copper grids (spi Supplies, 3530C-MB) under different experimental conditions. Silver NPs were deposited directly on the TEM grids under different experimental conditions. Particle size distributions were obtained from TEM images using ImageJ.
6
Transmission Electron Microscopy of Fossil Feathers
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Fossil feather material was removed from S1 using a sterile scalpel and placed in pure alcohol. The alcohol was then replaced with acetone, and stepwise substituted with epoxy resin (AGAR 100 Resin kit, R1031) to fully infiltrate the remnant tissues. The epoxy was left to polymerise at 60 °C for 48 h. Infiltrated sub-samples were trimmed with a razor blade and then 1.5 μm thick sections were cut using a glass knife mounted on an ultrotome (Leica Ultracut UCT). A diamond knife was employed for the ultra-thin sectioning at 50 nm, after which slices were fixed to pioloform-coated copper grids. These were inserted into a JEOL JEM-1230 transmission electron microscope run at 80 kV. Areas of interest were photographed using a Gatan MultiScan 794 CCD camera.
7
3D Reconstruction of PufX Mutant Complexes
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The purified core complexes from PufX R49/53L and PufX− mutants were used for 3D EM single particle reconstruction. Protein solutions were diluted to an A874 of 1.0 prior to preparation of TEM grids and negative staining. Five μl protein solution were applied on the surface of a carbon coated 400 mesh Cu grid, which was glow discharged for 30 s before use. Excess protein solution was blotted away by touching the edge of the grid with filter paper. The grid was then washed twice with water, stained with 0.75% uranyl formate for 30 s, then dried in air. All images were recorded at room temperature using a Philips CM-100 microscope equipped 1 K × 1 K Gatan Multiscan 794 CCD camera. Magnification was set at 61,000 X corresponding to 3.93 Å per pixel at the specimen level. The defocus value was varied between ~ 0.5 to 1.5 μm.
8
Negative Staining for Electron Microscopy
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A volume of glutaraldehyde (Sigma-Aldrich) corresponding to a final concentration of 0.075% was added to the protein samples followed by incubation overnight at 4 °C before grid preparation. Negative staining samples were prepared using the sandwich carbon method with home-made carbon film and uranyl formate or uranyl acetate (2%) [41 (link)]. The images were taken in a Tecnai T12 electron microscope (FEI, Eindhoven, The Netherlands) with a Multiscan 794 CCD camera (Gatan, Pleasanton, U.S.A.) operated at 120 kV at a nominal magnification of 52,000 × , which corresponded to an apparent magnification of 63,160x. The pixel size on the specimen level was 3.8 Å/pixel.
9
Visualization of Vps1 Oligomers
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10 µl of 1 µM purified unspun Vps1 oligomers were visualized by negative
staining. Samples were adsorbed on glow discharged carbon-coated copper
grids and stained with 0.75 % uranyl formate. Electron micrographs were
recorded on a Philips CM100 electron microscope using a Gatan MultiScan 794
CCD camera.
staining. Samples were adsorbed on glow discharged carbon-coated copper
grids and stained with 0.75 % uranyl formate. Electron micrographs were
recorded on a Philips CM100 electron microscope using a Gatan MultiScan 794
CCD camera.
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