Hydrogen peroxide h2o2 assay kit

Paclitaxel was obtained from National Institute for Food and Drug Control. Soybean phospholipids (SPC) was purchased from Kelong Chemical (Chengdu, China). Cholesterol (Chol) was purchased from Bio Life Science & Technology Co., Ltd (Shanghai, China). D-(+)-Biotin was purchased from shanghai darui fine chemical Co., Ltd (Shanghai, China). GOx was obtained from Sigma-Aldrich (St. Louis, MO, USA). 1,2-dioleoyl-snglycero-3-phosphoethanolamine-N-(carboxyfluorescein) (CFPE) was purchased from Avanti Polar Lipids (USA). 4'-6-Diamidino-2-phenylindole (DAPI) and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Beyotime Institute Biotechnology (Haimen, China). 4-chlorobenzenesulfonate salt (DiD) was purchased from Biotium (USA). Annexin V-FITC/PI apoptosis detection kit was obtained from BD Pharmingen^TM (USA). Enhanced BCA Protein Assay Kit (Beyotime^®, China). Hydrogen Peroxide (H₂O₂) Assay Kit (Beyotime®, China). Reactive Oxygen Species (ROS) Assay Kit (Beyotime®, China). Calcein-AM/PI Live/Dead Cell Double Dyed Kit (Solarbio®, China). JC-1 dye (Beyotime®, China).

In order to maintain the surface atoms of samples equivalent, different concentrations of Pd nanocrystals dispersions (1.50 mg·ml⁻¹ for cubes and 0.54 mg·ml⁻¹ for octahedrons) were prepared. For the oxidation of AA, the Pd nanocrystals dispersions were added into 0.1 M AA solution and then UV-Vis absorption spectra was recorded at different times using an UV spectrometer (UV-3600, Shimadzu, Japan).
For the identification of the product of catalytic oxidation, a hydrogen peroxide (H₂O₂) assay kit (Beyotime Institute of Biotechnology, Shanghai, China) was employed, which is based on the oxidation of ferrous ions (Fe²⁺) to ferric ions (Fe³⁺) by peroxides and the Fe³⁺ then combine an dye-xylenol orange to form a purple colored complex with the maximum absorbance at 560 nm measurable. In brief, the reaction solution was centrifuged at 14,800 r.p.m. for 15 min and the supernatants were collected. Finally, the surpernatants and detection solution were mixed for 30 min at room temperature and then measured using a microplate reader (BioTek, Synergy NEO, USA). The level of H₂O₂ in products was calculated according to a standard concentration curve with triplicate experiments.

Iron (III) chloride hexahydrate (FeCl₃·6H₂O), sodium oleate, oleic acid, 1-octadecene, poly (allylamine hydrochloride) (PAH; M_w = 15,000 Da), phosphate buffered saline (PBS), tetramethylammonium hydroxide, N-(3-(dimethylamino)propyl-N′-ethylcarbodiimide) hydrochloride (EDC), N-hydroxysulfosuccinimide sodium salt (NHS), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H-tetrazolium bromide (MTT) assay kit and 2ʹ,7ʹ-dichlorohydrofluorescein diacetate (DCFH; ≥ 94%), glucose oxidase (GOx) were purchased from Sigma-Aldrich. 3,3′,5,5′-tetramethylbenzidine (TMB), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), o-phenylenediamine (OPD; 98%), methylene blue (MB), terephthalic acid (TA) and hydrogen peroxide (H₂O₂) assay kit were obtained from Beyotime Biotechnology. Bicinchoninic acid (BCA) protein assay kit, malondialdehyde (MDA) and protein carbonyl assay kit were obtained from Nanjing Jiancheng Institute of Biological Engineering. Live/dead bacteria viability kit was purchased from Thermo Fisher Scientific. All other chemicals were obtained from Adamas-beta and used without further purification. Deionized (DI) water (Millipore Milli-Q grade, 18.2 MΩ) was used in all the experiments.