Antibodies against β-actin (#66009-1-Ig), IL-1β (#16806-1-AP), IL-6 (#66146-1-Ig), and Fibronectin (#15613-1-AP), as well as goat anti-rabbit (#SA00001-1) and goat anti-mouse (#SA00001-15) secondary antibodies were purchased from Proteintech (Rosemont, IL, USA). Antibodies against TSC1 (#6935), p-AMPK Thr-172 (#2535), AMPK (#5832), p-Akt Ser-473 (#4060), p-Akt Thr-308 (#13038), Akt (#2920), PCNA (#2586), Caspase-3 and Cleaved caspase-3 (#9664), BAD (#9239), BAX (#5023), F4/80 (#30325), CD3 (#99940), CD4 (#25229), and α-SMA (#19245) were purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit anti-Ki-67 (#15580) was purchased from ABCAM. Fluorescein-labeled LTL (#FL-1321) and Dylight 594 anti-rabbit IgG (#DI-1594) were purchased from Vector Labs. Peroxidase marker and DAB + Substrate (#5007) were purchased from Dako.
Dylight 594 anti rabbit igg
Manufactured by Vector Laboratories
Sourced in United States
DyLight 594 anti-rabbit IgG is a secondary antibody conjugated with a fluorescent dye. It is designed to detect and visualize rabbit primary antibodies in various immunoassays and imaging applications.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti ki67, by Abcam (1 mentions)
Dab substrate, by Agilent Technologies (1 mentions)
β actin 66009 1 ig, by Proteintech (1 mentions)
α sma, by Cell Signaling Technology (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions)
Normal goat serum (ngs), by Vector Laboratories (1 mentions)
Ethylenediaminetetraacetic acid, by Merck Group (1 mentions)
Phenylmethylsulfonyl fluoride pmsf, by Merck Group (1 mentions)
Human tnf α, by Merck Group (1 mentions)
Mounting medium, by Vector Laboratories (1 mentions)
Alexa fluor 488 goat anti mouse igg antibody, by Thermo Fisher Scientific (1 mentions)
Vectashield antifade mounting medium with dapi, by Vector Laboratories (1 mentions)
Yap taz, by Cell Signaling Technology (1 mentions)
Tcs sp8, by Leica (1 mentions)
Chamber slide, by Merck Group (1 mentions)
Dylight 488 anti mouse igg, by Vector Laboratories (1 mentions)
Prolong gold anti fade with 4 6 diamidino 2 phenylindole, by Thermo Fisher Scientific (1 mentions)
Confocal microscope, by Zeiss (1 mentions)
5 protocols using dylight 594 anti rabbit igg
1
Immunohistochemical Analysis of Signaling Pathways
2
Immunofluorescent Staining of Cultured Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Culture media M199, fetal bovine serum (FBS), and antibiotics were purchased from Gibco (Carlsbad, CA, USA). Normal goat serum, mounting medium, and Dylight® 594 anti-rabbit IgG were purchased from Vector Laboratories (Burlingame, CA, USA). Alexa Fluor 488 goat anti-mouse IgG antibody was acquired from Molecular Probes (Eugene, OR, USA). Rabbit polyclonal antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Human TNF-α, ethylenediaminetetraacetic acid, ethylene glycoltetraacetic acid, phenylmethylsulfonyl fluoride (PMSF), and Triton X-100 were purchased from Sigma (St. Louis, MO, USA). The primary antibodies used in this study are summarized in Table 1 . Oleracones D, E, and F were synthesized in Professor Han’s laboratory as previously described [33 (link)].
3
Immunohistochemical Analysis of Skin Tumors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Skin tumor samples were harvested from mice 42 days post induction. Tumor samples were fixed with formalin and subsequently paraffin embedded. Tissue was cut into 5μm slices and rehydrated through an ethanol to water wash series. Antigen retrieval was performed by boiling the sections in Tris-EDTA buffer for 30 minutes and subsequently placed in cold ddH2O for 10 minutes. Sections were blocked using 5% normal horse serum in 1X TBS and probed for YAP/TAZ (Cell Signaling, 1:200) at 4 degrees Celsius overnight. Sections were washed with 1X TBS-T and incubated with DyLight 594 Anti-Rabbit IgG (diluted in blocking buffer 1:200, Vector Labs) at room temperature for an hour. Three additional washes with washing buffer for 5 minutes were performed prior to mounting with Vectashield Anti-fade Mounting Medium with DAPI (Vectashield).
4
Immunofluorescent Staining of EphA2 and Shp2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunofluorescent staining was performed as described previously by us43 (link). Briefly, cells were plated into chamber slides (Millipore) and fixed in 4% paraformaldehyde, permeabilized, and incubated with mouse anti-EphA2 antibody (1 : 300 dilution) and rabbit anti-Shp2 antibody (1 : 100 dilution), followed by incubation with DyLight® 488 anti-mouse IgG (DI-2788, Vector Laboratories) and DyLight® 594 anti-Rabbit IgG (DI-1794, Vector Laboratories). Images were captured using an inverted confocal fluorescent microscope (LEICA TCS SP8). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI).
5
Immunofluorescence Analysis of HIF1α and ZEB2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells cultured on coverslips were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% bovine serum albumin (BSA) in 1× PBS. Cells were incubated with primary antibodies for HIF1α (1:100) and ZEB2 (1:100). HIF1α and ZEB2 were visualized using Cy5 horse anti-mouse IgG (1:200) and DyLight594 anti-rabbit IgG (1:200), respectively (Vector Laboratories, Burlingame, CA). ProLong gold antifade with 4′,6-diamidino-2-phenylindole is used to mount the slides (Thermo Fisher Scientific, Waltham, MA). Images were acquired using a confocal microscope (ZEISS, Thornwood, NY) at ×63 magnification. Similarly, expression of HIF1α and ZEB2 in glomerular sections of experimental rats were analyzed using a trinocular microscope (Leica, Buffalo Grove, IL).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!