Epyrifenacil and S-3100-CA were synthesized in our laboratory. The chemical purity of these compounds was determined to be 96.8% and 99.7% (HPLC), respectively. Phenyl-14 C-labeled S-3100-CA was also synthesized in our laboratory and had a specific activity of 4.26 GBq/mmol with radiochemical purity of 97.8% (radio-HPLC). The structures of these compounds are shown in Fig. 1. α-Cyano-4-hydroxycinnamic acid (α-CHCA) was supplied by Merck KGaA (Darmstadt, Germany). PPIX analytical standard was supplied by Frontier Scientific (Logan, UT, USA). Rifampicin was supplied by FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Digoxin was supplied by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Krebs-Henseleit buffer (KHB), silicone oil, and mineral oil were purchased from Merck KGaA. The hepatocyte isolation kit was supplied by Sekisui XenoTech, LLC (Kansas City, KS, USA). Cryopreserved primary hepatocytes of male SD rats (pool of 8), female SD rats (pool of 12), male CD-1 mice (pool of 16), and female CD-1 mice (pool of 23) were from Sekisui XenoTech, LLC. Cryopreserved primary hepatocytes of humans (pool of 10 males and 10 females) were purchased from Biopredic International (Saint Grégoire, France). Other chemicals were of reagent grade.
α cyano 4 hydroxycinnamic acid α chca
Manufactured by Merck Group
Sourced in United Kingdom, United States, Germany
α-cyano-4-hydroxycinnamic acid (α-CHCA) is a chemical compound commonly used as a matrix for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It is a crystalline solid that facilitates the ionization of analytes, allowing their detection and analysis by mass spectrometers.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Fujifilm (2 mentions)
Formic acid, by Fujifilm (2 mentions)
2 propanol, by Fujifilm (2 mentions)
Trifluoroacetic acid, by Merck Group (2 mentions)
2 5 dihydroxybenzoic acid dhb, by Merck Group (2 mentions)
Acetone, by Thermo Fisher Scientific (2 mentions)
Trifluoroacetic acid tfa, by Merck Group (2 mentions)
Acetonitrile acn, by Thermo Fisher Scientific (2 mentions)
Silicone oil, by Merck Group (1 mentions)
Mineral oil, by Merck Group (1 mentions)
Krebs henseleit buffer, by Merck Group (1 mentions)
Female cd 1 mice, by Sekisui (1 mentions)
Rifampicin, by Fujifilm (1 mentions)
Gelatin, by Merck Group (1 mentions)
2 5 dihydroxybenzoic acid, by Merck Group (1 mentions)
Acetonitrile, by Kanto Chemical (1 mentions)
Bovine insulin, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Ammonium hydroxide, by Merck Group (1 mentions)
13 protocols using α cyano 4 hydroxycinnamic acid α chca
1
Synthesis and Purity Analysis of Epyrifenacil and S-3100-CA
2
Analytical Protocol for Azoxystrobin
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Gelatin, α-cyano-4-hydroxycinnamic acid (α-CHCA), and 2,5-dihydroxybenzoic acid (DHB) were purchased from Merck (Darmstadt, Germany). Methanol, an azoxystrobin standard, formic acid, and 2-propanol were purchased from FUJIFILM Wako Pure Chemicals (Osaka, Japan). Acetonitrile was purchased from Kanto Chemical Co. (Tokyo, Japan). Ultrapure water was prepared using an ultrapure water apparatus (GenPure XCAD UV-TOC, Thermo Fisher Scientific, Waltham, MA, USA).
3
Amyloid-beta Peptide Synthesis
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Lyophilized Aβ1–42 and Aβ1–16 peptide were purchased from rPeptide (Watkinsville, GA, USA). Bovine insulin, DEPC; 1,1,1,3,3,3‐hexafluoro‐2‐propanol (HFIP); sodium chloride; sodium hydroxide; ammonium hydroxide; sodium phosphate monohydrate; disodium phosphate heptahydrate; α‐cyano‐4‐hydroxycinnamic acid (α‐CHCA); acetonitrile; trifluoroacetic acid; thioflavin T (ThT); and hydroxylamine hydrochloride (NH2OH·HCl) were purchased from Sigma‐Aldrich (St. Louis, MO, USA).
4
Trypsin Digestion of Coomassie-Stained Proteins
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The bands detected using Coomassie brilliant blue‐G250 were collected and incubated with 30% acetonitrile in 0.1% trifluoroacetic acid until the color disappeared. We then modified the proteins through alkylation with 10 mm dithiothreitol and 50 mm monoiodoacetic acid. The modified proteins were digested overnight with 10 ng·μL−1 N‐tosyl‐l ‐phenylalanine chloromethyl ketone‐trypsin (Sigma‐Aldrich) in 100 mm ammonium bicarbonate at 37 °C. For desalting, we loaded the digested proteins into ZipTip C18 pipette tips (Millipore, Billerica, MA, USA) and eluted them with 0.1% trifluoroacetic acid (TFA)/acetonitrile (1 : 2, v/v). Next, we performed mass spectrometry on a Bruker Autoflex (Bruker Daltonics, Bremen, Germany). We mixed the digested samples with an equal volume of saturated α‐cyano‐4‐hydroxycinnamic acid (α‐CHCA) (Sigma‐Aldrich) in 0.1% TFA/acetonitrile (1 : 2, v/v) and applied them to a target plate. We obtained the spectra in the positive mode and analyzed them with FLEXAnalysis (Bruker Daltonics). We used the Mascot program (Matrix Science, London, UK) to conduct database searches.
5
Atorvastatin Quantification in Porcine Intestine
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Gibco BenchStable DMEM/F12, phosphate-buffered saline (PBS), 2-methylbutane 99+% extra pure, LC-Grade methanol, LC-Grade acetonitrile (ACN), and acetone were purchased from Fisher Scientific Ltd. (Loughborough, UK). Atorvastatin calcium, α-Cyano-4-hydroxycinnamic acid (α-CHCA) and 2,5-Dihydroxybenzoic acid (DHB) were purchased from Sigma Aldrich (Dorset, UK). The deuterated internal standard, Atorvastatin-(anilide ring-d5) calcium salt was purchased from Merck Life Sciences (Dorset, UK). Formic acid 98% was purchased from Scientific Laboratory Supplies (Nottingham, UK). In total, 18.2 MΩ × cm water was collected from an ELGA water purification system (Buckinghamshire, UK). Cryo-M-Bed was purchased from VWR International Ltd. (Lutterworth, UK). Porcine small intestine was provided by R.B Elliott & Son (Chesterfield, UK). Super refined Polysorbate (80) LQ was donated by CRODA (New Castle, DE, USA).
6
Characterization of Chlorinated Benzoquinones
7
Quantification of Bevacizumab and Erlotinib
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Bevacizumab was obtained from F. Hoffmann-La Roche (Basel, Switzerland). Human immunoglobulin G (human IgG) was purchased from MP Biomedicals (Santa Ana, CA, USA). Erlotinib for animal experiments was obtained from F. Hoffmann-La Roche. Erlotinib for LC-MS/MS analysis was purchased from Selleck Chemicals (Houston, TX, USA). Erlotinib D6 (stable isotope–labelled internal standard for Erlotinib) was purchased from Toronto Research Chemicals (North York, ON, Canada). α-Cyano-4-hydroxycinnamic acid (α-CHCA) and 2,5-dihydroxybenzoic acid (DHB) were purchased from Sigma-Aldrich (St. Louis, MO, USA). HPLC grade trifluoroacetic acid (TFA), and LCMS grade methanol (for LC-MS/MS) were purchased from Kanto Chemical Co. (Tokyo, Japan). Formic acid, LCMS grade acetonitrile, and acetone were purchased from Wako Pure Chemical Industries (Osaka, Japan). The Qubit kit was purchased from Thermo Fisher Scientific.
8
Expression of Epitope-Tagged DDHD1 Mutant
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Oligonucleotides for the expression of the epitope-tagged DDHD1 mutant in HEK293 and PNAC1 cells described below were synthesized by Invitrogen. Sequencing-grade modified trypsin and AspN were purchased from Promega. High-purity MS grade 2, 5-dihydroxybenzoic acid (DHB) was from Shimadzu. α-Cyano-4-hydroxycinnamic acid (α-CHCA) was from Sigma. Phos-tag acrylamide was from Wako. The mixture of protease inhibitors containing 4-(2-aminoethyl) benzenesulfonyl fluoride, aprotinin, bestatin, E-64, leupeptin, and pepstatin A was from Calbiochem. Olomoucine was from Sigma. CHIR99021 was from Wako. Anti-mouse IgG antibodies conjugated with horseradish peroxidase were from Dako. All other reagents were from Wako, unless otherwise mentioned. All other compounds were of reagent grade or higher. The expression plasmid for paxillin α (35 (link)) was a generous gift of Dr M. Nishita (Fukushima Medical University).
9
Characterization of AZD2811 Nanoparticles
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AZD2811 nanoparticles were provided by AstraZeneca. AZD2811 (cat no. SML0268) and α-cyano-4-hydroxycinnamic acid (α-CHCA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 2,5-Dihydroxybenzoic acid (DHB), formic acid (FA), trifluoroacetic acid (TFA), acetonitrile, methanol, 2-propanol, Mayer’s hematoxylin solution, and 1% eosin Y solution for H&E staining were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan). Masson’s trichrome staining kits were purchased from Polysciences, Inc. (Warrington, PA, USA). Reagents for immunohistochemistry were purchased from Cell Signaling Technology (Beverly, MA, USA), Abcam (Cambridge, UK), BD Biosciences (Franklin Lakes, NJ, USA), and Dako (Santa Clara, CA, USA).
10
Fingermark Development Materials Sourcing
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Acetonitrile (ACN) and acetone were obtained from Fisher Scientific (Loughborough, UK). Trifluoroacetic acid (TFA) and α-cyano-4-hydroxycinnamic acid (α-CHCA) were purchased from Sigma Aldrich (Poole, UK). The materials used for fingermark development; cyanoacrylate (fuming) (CAF), basic yellow 40 (BY40), black powder suspension (BPS) and Basic Violet 3 (BV3) were provided by West Yorkshire Police (WYP) (Wakefield, UK). Brown (parcel) tape was purchased from Sainsbury's (Sheffield, UK) whereas clear tape was provided by the Fingerprint Enhancement Laboratory (FEL), Yorkshire and Humber Regional Scientific Support Services (YHRSSS) (Wakefield, UK).
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