Spss statistics 22 24

Statistical evaluation of the qRT-PCR (Figure 3) and the western blot analysis (Figure 4) of different brain regions in sham-treated and treated Npc1^+/+ and Npc1^-/- mice was carried out with a nonparametric Mann–Whitney U-test using SPSS statistics 22/24 (IBM, Chicago, IL, USA). Equally, this test was used for statistical analysis of patient-derived fibroblasts (Figure S6). Statistical analysis of the corpus callosum diameter was performed with GraphPad Prism 7 using one-way analysis of variance (ANOVA) followed by Bonferroni correction of multiple comparisons. All graphs and heat maps were created using GraphPad Prism 7 (GraphPad Software, San Diego, CA, USA). Data are given as mean ± SEM and considered significant if p ≤ 0.05 (* p ≤ 0.05, *** p ≤ 0.001).

Statistical evaluation of the olfactory behavior test, the cell quantifications and the qRT-PCR was done with a non-parametric Mann-Whitney U-test by SPSS statistics 22/24 (IBM, Chicago, IL, United States) using genotype and treatment groups as independent variables. Graphs are created using GraphPad Prism 7. p ≤ 0.05 was considered significant.