H3k4m3

ChIP assay was performed according to the protocol developed by Upstate Biotechnology as described (Meng et al., 2013 (link)). Briefly, treated myotubes were fixed with 1% formaldehyde and sonicated to produce chromatin lysates. The lysates were pre-cleared with Protein-G agarose beads and immunoprecipitated overnight with antibodies against Ace-H3 (Millipore, 06–599), H3K4m3 (Millipore, 07–473), H3K9m2 (Abcam, ab1220), or control IgG in the presence of BSA and salmon sperm DNA. The next day, Protein-G agarose beads were added to each immunoprecipitation reaction for 1 h, followed by extensively wash and reverse crosslink. DNA was eluted from the beads and purified using a PCR Purification Kit (Invitrogen) and subsequently analyzed by qPCR using primers located on the proximal promoter regions of mouse Baf60c and Deptor genes (Table S1).

HEK293T cells were transiently transfected with HA-CAR and Myc-Baf60a for 24 h. Total lysates or immunoprecipitated proteins were analyzed by western blot using antibodies against c-Myc (Sigma, C3956; 1:2,000) or HA (Santa Cruz, sc-66181; 1:2,000). ChIP assay was performed according to the protocol developed by Upstate Biotechnology as described (Meng et al., 2014 (link)). Briefly, liver nuclei or primary hepatocytes were fixed with 1% formaldehyde and sonicated to produce chromatin lysates. The lysates were pre-cleared with Protein-G agarose beads and immunoprecipitated overnight with antibodies against Ace-H3 (Millipore, 06-599), H3K4m3 (Millipore, 07-473), Brg1 (Santa Cruz, sc-17796×), or control IgG in the presence of BSA and salmon sperm DNA. The next day, Protein-G agarose beads were added to each immunoprecipitation reaction for 1 h, followed by extensively wash and reverse crosslink. DNA was eluted from the beads and purified using a PCR Purification Kit (Invitrogen) and subsequently analyzed by qPCR using primers located on the proximal CAR promoter. Primers are available upon request.