Ds camera control unit ds u2

The abalone shell was gently removed by severing the adductor muscle as close to the shell as possible using a thin metal spatula, without rupturing the digestive tract. The animals were placed adductor muscle side down in labeled embedding cassettes and fixed in Davidson’s solution [24] (per litre (vol/vol): 330 ml 95% ethanol (Merck), 220 ml 100% formalin (Merck), 115 ml glacial acetic acid (Merck) and 335 ml distilled water) for 36 hours at 4°C, before being transferred to 70% (vol/vol) ethanol at 4°C. Following fixation, the abalone samples were dehydrated through a graded ethanol series to 100% xylene (Saarchem) in a Tissue Trek II tissue processor. The dehydrated tissue samples were embedded in paraffin wax, sectioned at 5 µm, adhered onto positively charged microscope slides (SuperFrost® Plus, Menzel-Gläser) and stored in slide boxes at room temperature.
The H. midae sections were deparaffinised and stained using standard Harris’ H&E stain according to Hayat [25] and mounted with phosphate-buffered glycerin jelly [50% (vol/vol) Glycerol (Merck), 0.5 M phosphate buffer (pH 7.0) and 7.5% (wt/vol) gelatine (Merck)]. The sections were viewed using a Nikon Eclipse 50 i Compound Microscope equipped with a Nikon DS Camera Control Unit DS-U2 and DS-5M Camera head with Nikon Software (NIS Elements Documentation and Digital 3D Imaging).

Samples were sectioned into 6‐μm‐thick cross‐sections using a microtome and stained with Toluidine Blue O. Sections were examined at × 40 and × 100 (oil) using a Nikon Eclipse 50i Compound Microscope (Nikon, Tokyo, Japan). A Nikon DS Camera Control Unit DS‐U2 and DS‐5M Camera Head were used to capture images, which were then processed using NIS Elements Documentation software (Nikon).