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Pvegfr2 y1059

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PVEGFR2 Y1059 is a laboratory reagent used in research applications. It is a phospho-specific antibody that recognizes the VEGFR2 protein phosphorylated at tyrosine residue 1059. This reagent can be used for the detection and analysis of VEGFR2 phosphorylation in various experimental procedures, such as Western blotting, immunoprecipitation, and immunocytochemistry.

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Lab products found in correlation

Pvegfr2 y1059, by Cell Signaling Technology (2 mentions) Pvegfr2 y951, by Cell Signaling Technology (2 mentions) Total vegfr2, by Cell Signaling Technology (2 mentions) Ve cad, by Santa Cruz Biotechnology (2 mentions) Sc 9989, by BD (2 mentions) Ve cad, by BD (2 mentions) Sc 1718 r, by BD (2 mentions) Goat anti vegfr2, by R&D Systems (2 mentions) Rabbit anti ve cadherin, by Cell Signaling Technology (2 mentions) Pvegfr2 y1175, by Cell Signaling Technology (2 mentions) Rabbit anti rab5, by Cell Signaling Technology (2 mentions) Rabbit anti ha, by Cell Signaling Technology (2 mentions) Ha tag, by Cell Signaling Technology (2 mentions) Mouse anti ha 11, by Fortrea (2 mentions)

2 protocols using pvegfr2 y1059

1

Antibody Panel for Vascular Signaling Analysis

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List of antibodies with application and dilutions (IHC, immunohistochemistry; WB; western blot): pVEGFR2 Y1175 (WB 1:1000, Cell Signaling #2478), pVEGFR2 Y1059 (for HUVEC, WB 1:1000, Cell Signaling #3817, ), pVEGFR2 Y1059 (for mouse ECs, WB 1:1000, EMD Millipore #ABS553), pVEGFR2 Y951 (WB 1:1000, Cell Signaling #4991 ), VEGFR2 total (WB 1:1000, Cell Signaling #2479), VE-Cad (for HUVEC, WB 1:200, Santa Cruz #sc-9989 (F-8) ), VE-Cad (for mouse ECs, WB 1:500, BD Pharmingen #555289), mouse Sdc2 (WB 1:200, R&D #AF6585 - polyclonal raised against mouse Sdc2 ED), human Sdc2 (WB 1:200, R&D # AF2965, polyclonal raised against mouse human ED), mouse PTP1b (WB 1:200, Santa Cruz #sc-1718-R (N19-R)), human PTP1b (WB 1:500, BD Pharmingen #610139), VEPTP ( VE-PTP-C pAb69 and VE-PTP 1–8 pAb70 were gifts from Prof. Dietmar Vestweber, Max Plank Institute for Molecular Biomedicine, Münster, Germany), DEP1 (WB 1:200, Santa Cruz #sc-21761 (143–41)) NRP1 (WB 1:1000, Cell signaling #3725,), HA-tag (WB 1:1000, Cell signaling #3724), rabbit anti-Rab5 (Cell Signaling, #3547, IHC 1:200); goat anti-VEGFR2 (R&D, #AF357, IHC 1:100); mouse anti-HA.11 (Covance, #MMS-101P, IHC 1:200); rabbit anti-HA (Cell signaling, #3724, IHC 1:200); rabbit anti-VE-Cadherin (Cell Signaling, #2500, IHC 1:200); goat anti-mouse VE-Cadherin (R&D Systems, #AF1002, IHC 1:100); rat anti-mouse Flk1 (BD, #555307, IHC 1:100).
Corti F., Ristori E., Rivera-Molina F., Toomre D., Zhang J., Mihailovic J., Zhuang Z, & Simons M. (2022). Syndecan-2 selectively regulates VEGF-induced vascular permeability. Nature cardiovascular research, 1(5), 518-528.
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2

Antibody Panel for Vascular Signaling Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
List of antibodies with application and dilutions (IHC, immunohistochemistry; WB; western blot): pVEGFR2 Y1175 (WB 1:1000, Cell Signaling #2478), pVEGFR2 Y1059 (for HUVEC, WB 1:1000, Cell Signaling #3817, ), pVEGFR2 Y1059 (for mouse ECs, WB 1:1000, EMD Millipore #ABS553), pVEGFR2 Y951 (WB 1:1000, Cell Signaling #4991 ), VEGFR2 total (WB 1:1000, Cell Signaling #2479), VE-Cad (for HUVEC, WB 1:200, Santa Cruz #sc-9989 (F-8) ), VE-Cad (for mouse ECs, WB 1:500, BD Pharmingen #555289), mouse Sdc2 (WB 1:200, R&D #AF6585 - polyclonal raised against mouse Sdc2 ED), human Sdc2 (WB 1:200, R&D # AF2965, polyclonal raised against mouse human ED), mouse PTP1b (WB 1:200, Santa Cruz #sc-1718-R (N19-R)), human PTP1b (WB 1:500, BD Pharmingen #610139), VEPTP ( VE-PTP-C pAb69 and VE-PTP 1–8 pAb70 were gifts from Prof. Dietmar Vestweber, Max Plank Institute for Molecular Biomedicine, Münster, Germany), DEP1 (WB 1:200, Santa Cruz #sc-21761 (143–41)) NRP1 (WB 1:1000, Cell signaling #3725,), HA-tag (WB 1:1000, Cell signaling #3724), rabbit anti-Rab5 (Cell Signaling, #3547, IHC 1:200); goat anti-VEGFR2 (R&D, #AF357, IHC 1:100); mouse anti-HA.11 (Covance, #MMS-101P, IHC 1:200); rabbit anti-HA (Cell signaling, #3724, IHC 1:200); rabbit anti-VE-Cadherin (Cell Signaling, #2500, IHC 1:200); goat anti-mouse VE-Cadherin (R&D Systems, #AF1002, IHC 1:100); rat anti-mouse Flk1 (BD, #555307, IHC 1:100).
Corti F., Ristori E., Rivera-Molina F., Toomre D., Zhang J., Mihailovic J., Zhuang Z, & Simons M. (2022). Syndecan-2 selectively regulates VEGF-induced vascular permeability. Nature cardiovascular research, 1(5), 518-528.
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