Atcc crl 1619

The MM cell line A375 (ATCC^® CRL-1619™) was purchased from American Type Culture Collection (ATCC, USA). The culture medium for A375 cells was Dulbecco's modified Eagle's medium (DMEM, ATCC, Cat. No. 30-2002) supplemented with 10% fetal bovine serum (FBS, Gibco, USA). The cells were maintained in the environment with 5% CO₂ and 37°C.
SchA (≥98.0% (HPLC), Figure 1) was obtained from Sigma-Aldrich (USA). SchA was diluted in dimethylsulfoxide (DMSO) to 0–50 μM. The cells were treated with SchA for 24 h.

HaCaT (non-tumoral, CLS 300493, purchased from CLS HaCaT/300493">https://cls.shop/HaCaT/300493, accessed on 21 June 2023), A375 (melanoma, ATCC CRL-1619, purchased from ATCC https://www.atcc.org/products/crl-1619, accessed on 21 June 2023), and A431 (epidermoid carcinoma CRL-1555, purchased from ATCC CRL-1555">https://www.atcc.org/products/CRL-1555, accessed on 21 June 2023) skin cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 5% fetal calf serum (FCS), 100 µg/mL of penicillin, and 100 µg/mL of streptomycin. The cells were maintained at 37 °C in a humidified incubator with a mixture CO₂/air (5%/95%). Before the experiments, cells were examined under a microscope for correct morphology, adherence, and exponential growth. For experiments, cells were seeded and cultured under standard conditions, after allowing the adhesion of cells, different concentrations of ΔM4 were added to cultures in the presence of complete medium (5% FCS). To evaluate the biological effect, cells were trypsinized, pelleted, and analyzed for different tests. All reported data include at least three independent experiments per treatment group.