Absorption spectra of phthalocyanines PcZr(L1)2, PcZr(L2)2, PcZr(L3)2 and ligands L1, L2, L3 with insulin were recorded on a SHIMADZU UV-VIS-NIR spectrophotometer UV-3600. Spectra were recorded at 250–820 nm region. Monomeric insulin and noninhibited insulin fibrils (after 300 min of reaction) at concentration of 340 μM were diluted in 20 times in 50mM Tris-HCl buffer (pH 7.9) and then the aliquot of stock solution (2 mM in DMSO) of corresponding compound (phthalocyanine or ligand) was added, so that the compound’s final concentration was 5 μM (thus the compound-to-protein concentrations ratio was the same as during the fibril formation reaction). Working solutions of phthalocyanines and ligands in methanol were also 5 μM. All spectral measurements were performed in quartz absorption cuvettes (1 ґ 1 cm) at room temperature.
Uv 3600 uv vis nir spectrophotometer
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu UV-3600 UV-vis-NIR spectrophotometer is a versatile instrument designed for absorption and transmission measurements across the ultraviolet, visible, and near-infrared spectral regions. It features high-resolution optics and advanced detector technology to provide accurate and reliable data.
Automatically generated - may contain errors
Lab products found in correlation
F 4600 fluorescence spectrophotometer, by Hitachi (4 mentions)
S 4800, by Hitachi (3 mentions)
Jem 2100f, by JEOL (3 mentions)
F 7000 fluorescence spectrophotometer, by Hitachi (3 mentions)
Escalab 250, by Thermo Fisher Scientific (3 mentions)
Multiskan sky, by Thermo Fisher Scientific (3 mentions)
K alpha xps system, by Thermo Fisher Scientific (3 mentions)
Polished two sided quartz cuvette, by Starna Cells (2 mentions)
Zetasizer nano zs90, by Malvern Panalytical (2 mentions)
Liquid state nmr, by Agilent Technologies (2 mentions)
Te2000 e, by Nikon (2 mentions)
Rf 5301 spectrofluorophotometer, by Shimadzu (2 mentions)
Tecnai g20, by Thermo Fisher Scientific (2 mentions)
Autoflex maldi tof ms, by Bruker (2 mentions)
Acf400 spectrometer, by Bruker (2 mentions)
Uv 2600 uv vis nir, by Shimadzu (2 mentions)
Micromass lct premier tof ms, by Waters Corporation (2 mentions)
Escalab 250 photoelectron spectrometer, by Thermo Fisher Scientific (2 mentions)
Escalab 250 xi xps system, by Thermo Fisher Scientific (1 mentions)
Tecnai f20, by Thermo Fisher Scientific (1 mentions)
82 protocols using uv 3600 uv vis nir spectrophotometer
1
Absorption Spectra of Phthalocyanine-Insulin Complexes
2
Comprehensive Characterization of Nanoparticle Formulations
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Transmission electron microscopy (TEM) analysis was performed on a transmission electron microscope (Hitachi HT7700, Japan). High-resolution transmission electron microscopy (HRTEM) images were obtained with a FEI Tecnai F20 (FEI, USA) at a voltage of 200 kV. The hydrodynamic size and zeta potential of PNPs and NMPNs were measured by Zetasizer Nano ZS90 (Malvern Instruments, UK) at room temperature. The X-ray photoelectron spectroscopy (XPS) results were obtained via a Thermo Scientific ESCALAB 250 Xi XPS system. X-Ray diffraction (XRD) patterns were performed by using an X-ray diffractometer (PANalytical B.V. X-pert Powder, Netherlands). Inductively coupled plasma mass spectrometry (ICP-MS, PerkinElmer NexION 300X, USA) was utilized to measure the concentration of Pt195 under the following test conditions: plasma gas flow rate was 17 L/min. The atomized gas flow rate was 1 L/min. The radio frequency (RF) power was 1300 W. The measured mode was KED mode (He gas flow rate: 3 mL/min). The integration time was 1000 ms. UV-vis absorption spectra were measured by a UV-Vis-NIR Spectrophotometer UV-3600 (Shimadzu, Japan). In vivo bioluminescence images were received by a VISQUE InVivo Elite imaging system. Catalase (CAT)-like activity assays of NMPNs was measured by an oxygen electrode on Multi-Parameter Analyzer (JPSJ-606L, Leici China).
3
Characterization of Ionic Molecular Dyes
4
Kinetic Study of Hydrogen Peroxide Oxidation
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UV–Vis analysis was conducted on Shimadzu UV–Vis-NIR Spectrophotometer UV-3600. In a 3 mL cuvette, an acetonitrile solution of 3 (0.04 mM, 2 mL) was scanned from 200–700 nm wavelength. 0.2 mL of 30% H2O2 (0.4 mM) was added into the cuvette and scanned immediately; then scanned again at time intervals of 5 min and 1 h. Subsequently, 0.2 mL of styrene was added to the cuvette mixture and monitored for 6 h.
5
Comprehensive Analytical Characterization Protocol
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Analytical thin-layer chromatographies were performed using aluminium-coated Merck Kieselgel 60 F254 plates. NMR spectra were recorded on a Bruker Avance 400 (1H: 400 MHz; 13C: 100 MHz) spectrometers at 298 K, using partially deuterated solvents as internal standards. Coupling constants (J) are denoted in Hz and chemical shifts (δ) in ppm. Electrospray ionization mass spectrometry and matrix-assisted laser desorption ionization (coupled to a time-of-flight analyzer) experiments were recorded on a HP1100MSD spectrometer and a Bruker REFLEX. TGA were performed using a TA Instruments TGAQ500 with a ramp of 10 °C min−1 under air from 100 to 1000 °C. TEM images were obtained with JEOL-JEM 2100F instrument or a JEOL-JEM GRAND ARM300cF (AC-HRTEM). AFM images were acquired using a JPK NanoWizard II AFM working in dynamic mode. NT-MDT NSG01 silicon cantilevers, with typical values of 5.1 N m–1 spring constant and 150 kHz resonant frequency, were employed under ambient conditions in air. TRXF analyses were performed on a TXRF 8030c - FEI Spectrometer. Raman spectra were acquired with a Bruker Senterra confocal Raman microscope instrument equipped with 532, 633, and 785 nm excitation lasers. UV-vis-NIR spectra were performed using a Shimadzu UV-VIS-NIR Spectrophotometer UV-3600. PLE intensity maps were obtained with NanoLog 4 HORIBA instrument.
6
Characterization of Ionic Molecular Dyes
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Samples of parent dyes (NBA2SO4, NaIR820), the IM ([NBA][IR820]), and the mixture of parent dyes (0.5 NBA2SO4:1 NaIR820) were prepared in ethanol. Since the IM contains NBA+ and IR820− in 1:1 ratio, a mixture was prepared by taking 0.5 mol eq. of NBA2SO4 with 1 mol eq. of NaIR820 to acquire 1:1 NBA+ and IR820− in the mixture. The Shimadzu UV-Vis-NIR spectrophotometer UV-3600 was used to study their absorption characteristics. A 10 mm path length, polished two-sided quartz cuvette (Starna Cells) was used for absorbance measurements against an identical cell filled with the solvent of the sample.
7
Characterization of Biomolecules and Microbes
8
Synthesis and Characterization of Au@Ag Nanoparticles
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Titanium dioxide (TiO2) (anatase) and tetrachloroauric(iii ) acid trihydrate (HAuCl4·3H2O) were purchased from Sigma Aldrich (USA) and Sisco Research Laboratories Pvt. Ltd. (India) respectively. Green tea extract was obtained from Fragrance Oils (International) Limited, UK. Methylene blue (MB) was obtained from HiMedia Laboratories (India). A Shimadzu UV-3600 UV-Vis-NIR spectrophotometer was used for all UV-visible spectroscopic and Localized Surface Plasmon Resonance (LSPR) measurements and centrifugation was performed using a Vision Scientific VS-15000N centrifuge. The hydrodynamic radii of the Au@Ag nanoparticles were determined via dynamic light scattering using a Malvern Nano-ZS zetasizer. Ultrapure water (conductivity < 0.05 μS cm−1) obtained from an Evoqua Water Technologies ultrapure water system was used in all synthesis, preparation, and experimental procedures.
9
Characterization of TiO2-VO2 Nanocomposite
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The morphology of the reaction product was examined by using scanning electron micros-copy (SEM, Hitachi S-4800). The phase identification of the TiO2 and VO2 films was performed using X-ray diffraction (XRD, Bruker-AXS diffractometer, Model D8 ANVANCE) with Cu-Kα radiation source, Raman spectra (HR800, excitation wavelength: 633 nm, laser power: 1 mW) and Transmission Electron Microscope (TEM, FEI Tecnai G2 F20 S-TWIN). The chemical valence of vanadium ions was measured by XPS (PHI QUANTERA-II SXM) with Al-Kα radiation source (1486.6 eV). The porosity based on SEM images was calculated by using Image-Pro Plus (IPP) to compare the gray scale pixel of the area occupied by VO2 nanoplates and exposed TiO2 films. The optical transmittance spectra of samples at normal incidence from 300 to 3000 nm and were measured by using Shimadzu UV-3600 UV-VIS-NIR spectrophotometer with Heat Solid Transmission Accessory.
10
Comprehensive Characterization of Graphene Aerogel
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The morphology of the GA-GH and GH was examined by field-emission scanning electron microscopy (FESEM) on a JSM-7500F scanning electron microscope (JEOL Ltd., Tokyo, Japan). The chemical states of the element on the graphene aerogel surface were investigated by X-ray photoelectron spectroscopy, (XPS) using an XSAM 800 photoelectron spectroscope (Kratos Analytical, Ltd, Manchester, U.K.). X-ray diffraction (XRD) spectra were recorded on a D/max-2200/PC X-ray diffractometer (Rigaku Corporation, Tokyo, Japan) with Cu Kα radiation. Thermogravimetric analysis (TGA) was performed using a TG209F1 Libra Thermogravimetric Analyzer (Netzsch NETZSCH Group, GmbH, Selb, Germany), from room temperature to 750 °C at a heating rate of 20 °C min−1 in air. The concentration of Cr(iii ) was determined by ICP-OES, using an Optima 2100DV spectrometer (PerkinElmer, Waltham, MA, USA). UV-vis diffuse reflectance spectra (UV-vis DRS) were recorded using a UV-3600 UV-vis-NIR spectrophotometer (Shimadzu, Kyoto, Japan) equipped with an integrating sphere and using BaSO4 as reference. The specific surface areas of GA-GH and GH were calculated by the Brunauer–Emmett–Teller (BET) method.
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