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High performance liquid chromatography solvents

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High performance liquid chromatography (HPLC) solvents are specialized chemical solutions used as the mobile phase in HPLC systems. They facilitate the separation and analysis of complex mixtures of compounds. HPLC solvents provide the necessary chemical properties to enable efficient and reliable chromatographic separations.

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2 protocols using high performance liquid chromatography solvents

1

Synthetic Lipid Reagents for Biophysical Studies

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Peptide synthesis reagents were purchased from AnaSpec (Fremont, CA, USA), high performance liquid chromatography (HPLC) solvents from Fisher Scientific (Pittsburgh, PA, USA), and all other chemicals from MilliporeSigma (St. Louis, MO, USA). Dipalmitoyl phosphatidylcholine (DPPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), and palmitoyl-oleoyl-phosphatidylglycerol (POPG) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). The clinical porcine surfactant Poractant Alfa (Curosurf®) was obtained from Chiesi Farmaceutici, Parma, Italy.
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2

Oligodeoxynucleotide Analysis by MALDI-TOF MS

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Oligodeoxynucleotides (ODNs) were purchased from Integrated DNA Technologies, Inc. (IDT) (Coralville, IA, USA). Nuclease P1 (NP1) from Penicillium citrinum was obtained from Sigma (St. Louis, MO, USA). Ammonium citrate and 3-hydroxypicolinic acid for use as matrix assisted laser desorption ionization (MALDI) matrices were purchased from Fluka (Milwaukee, WI, USA). High-performance liquid chromatography (HPLC) solvents were from Fisher (Fair Lawn, NJ, USA).
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