The samples were analyzed using inductively coupled plasma mass spectrometry (ICP-MS)with an iCAP Q instrument (Thermo Fisher Scientific), equipped with a Meinhard TQ + concentric quartz nebulizer, a Peltier-cooled high-purity quartz baffled cyclonic spray chamber, and a demountable quartz torch with a 2.5 mm i. d. quartz injector. The interface consisted of two Ni cones (sampler and skimmer). High-purity argon (99.9997%) was used as nebulizer and plasma gas. The sample solutions and calibration standards were introduced into the ICP-MS instrument using a CETAC ASX-520 autosampler (Teledyne CETAC Technologies). Before each run, the instrument was tuned for maximum sensitivity and signal stability to minimize the formation of oxides and double-charge ions. The main operational parameters of the ICP-MS were: nebulizer gas flow, 1.14 L/min; auxiliary gas flow, 0.79 L/min; plasma gas flow, 13.9 L/min; radiofrequency generator power, 1550 W; and dwell time, 1–10 m. The isotopes 7Li, 25Mg, 27Al, 31P, 43Ca, 52Cr, 55Mn, 57Fe, 59Co, 60Ni, 65Cu, 66Zn, 75As, 82Se, 85Rb, 88Sr, 111Cd, 121Sb, 133Cs, 137Ba, 205 TL and 208 Pb were measured for analytical determination and the isotopes 6Li, 45Sc, 71Ga, 89Y, 103Rh, 193Ir and 209Bi were monitored as IS (Azevedo et al., 2023 (link)).
Icap q instrument
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ICAP Q is an inductively coupled plasma mass spectrometry (ICP-MS) instrument manufactured by Thermo Fisher Scientific. It is designed for the analysis of trace elements in a wide range of sample types. The ICAP Q provides high-performance multi-element analysis with low detection limits and rapid sample throughput.
Automatically generated - may contain errors
Lab products found in correlation
Su8030, by Hitachi (1 mentions)
Icap 7600, by Thermo Fisher Scientific (1 mentions)
Nano z, by Malvern Panalytical (1 mentions)
Tristar 2 3020, by Micromeritics (1 mentions)
Uv 2600, by Shimadzu (1 mentions)
Smartlab tm 9 kw x ray diffractometer, by Rigaku (1 mentions)
Supra 55, by Zeiss (1 mentions)
6 protocols using icap q instrument
1
Trace Element Analysis by ICP-MS
2
Characterization of NU-1000 Metal-Organic Framework
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Crystallinity of each NU-1000 materials was verified with powder X-ray diffraction (PXRD) on a STOE STADI P with CuKα1 radiation and compared to a simulated NU-1000 pattern. Porosity of NU-1000 and cyt c@NU-1000 were measured using isothermal N2 adsorption at 77 K on Micromeritics Tristar II 3020. Pore size distributions (PSDs) were calculated by N2 DFT model with slit geometry. Prior to N2 adsorption measurements, cyt c@NU-1000 was activated with supercritical CO2 and at 80°C under vacuum overnight on Micromeritics Smart VacPrep instrument. UV-vis measurements were performed on Shimadzu UV-2600 in a quartz cuvette. SEM images and SEM-EDS line scans were taken on Hitachi SU8030 equipped with Oxford AZtec software after 9 nm of Os plasma coating. ICP-OES was performed on Thermo iCap7600, and ICP-MS was performed on Thermo iCapQ instrument. DLS and zeta potential were measured by Zetasizer (Malvern Instruments Ltd, NanoZS).
3
Quantification of Cellular Uptake of AgNPs
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Cellular uptake of the AgNPs was quantified using ICP-MS. After 3 and 6 weeks of exposure, cells were washed three times with PBS, harvested by trypsinization, re-suspended in cell medium and counted. The mineralization of the samples was performed in 4% HCl and 40% HNO3 for 48 h. Thereafter the samples were diluted to reach 2% HNO3 prior to the analysis. 107Ag and 109Ag isotopes were quantified using an iCAP Q instrument (Thermoscientific) running on KED mode. Calibration standards of 1, 5, 10, 50, 100, 500 ppb Ag were prepared using a 1000 ppm reference standard (Spectrascan). All samples were spiked with 5 ppb indium as an internal standard with a range of recovery between 85–105%. The limits of detection for the investigated isotopes were estimated at < 0.004 ppb for both isotopes. Each sample was injected at least 3 times and the relative standard deviation (RSD) acceptance was set at 10%. The average values of the analyzed isotopes were used and results were expressed as pg Ag/cell. For the Ag release in cell medium cell supernatants (4 days after exposure) were collected, spun down (15000 rpm, 30 min) and the upper fraction was mineralized and analyzed as described above.
4
Plasma Copper and Cardiovascular Outcomes
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During the first medical visit after study inclusion, at a median of 5.2 (IQR 1.8–12.1) years after transplantation, all participants provided fasting blood samples. Aliquots of plasma generated from these samples were stored frozen at −80 °C and plasma copper was afterwards measured using inductively coupled plasma mass spectrometry (ICPMS) ICAP Q instrument (Thermo Fisher Scientific, Waltham, MA, USA) at Synalb (Jena, Germany). Measurements were conducted following DIN EN ISO 17294-2: 2017-01 [31 (link)]. This test has a detection limit of 0.1 µg/L, an intra-assay variability of 0.3%, and inter-assay variability of 1%.
LDL cholesterol was calculated using the Friedewald equation. Estimated glomerular filtration rate (eGFR) was calculated using the serum creatinine-based Chronic Kidney Disease EPIdemiology collaboration equation (CKD-EPI). Cardiovascular mortality was defined as the principal cause of death being cardiovascular in nature (codes 410–447 of the International Statistical Classification of Diseases-9) [32 (link)]. Proteinuria was defined as urinary protein excretion ≥ 0.5 g/24 h.
LDL cholesterol was calculated using the Friedewald equation. Estimated glomerular filtration rate (eGFR) was calculated using the serum creatinine-based Chronic Kidney Disease EPIdemiology collaboration equation (CKD-EPI). Cardiovascular mortality was defined as the principal cause of death being cardiovascular in nature (codes 410–447 of the International Statistical Classification of Diseases-9) [32 (link)]. Proteinuria was defined as urinary protein excretion ≥ 0.5 g/24 h.
5
Determination of Plasma Zn and Cu
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Concentrations of Zn and Cu were measured from frozen plasma samples in 2022 by SGS Analytics Germany GmbH, via inductively coupled plasma–mass spectrometry ICAP Q instrument (Thermo Fisher Scientific, Waltham, MA, USA) in accordance with DIN EN ISO 17294-2: 2017-01 [22 ]. Prior to measurement, plasma sample aliquots were decomposed (nitric acid and hydrogen peroxide (4:1)) by microwave digestion [23 (link)]. Multi-element standard was used for calibration, while rhodium (2 µg/L) was added as internal standard. Spectroscopic interferences were minimized by use of a collision/reaction cell for detection.
6
Comprehensive Electrochemical and Spectroscopic Analysis
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Electrochemical data were obtained by an electrochemical analyzer (CHI760D, Shanghai, China) with a three-electrode system, containing a glassy carbon working electrode (Φ = 3 mm), a platinum electrode and a saturated calomel electrode (SCE) as a reference. The microscopic features of the samples were recorded on a scanning electron microscope (SEM, Zeiss Supra55, Heidenheim Germany) operated at an accelerating voltage of 5.00 kV. Transmission electron microscopy (TEM) images were recorded with an electron microscope (JEOL-1010, Tokyo, Japan) operated at 200 kV. Wide-angle X-ray diffraction (XRD) pattern was collected on a Rikagu Smartlab TM 9 KW X-ray diffractometer (Rigaku Corporation, Tokyo, Japan) in the 2θ from 10–80° using Cu Kα radiation. Inductively coupled plasma mass spectrometry (ICP-MS) analysis was obtained by a Thermo Scientific iCAP Q instrument (Waltham, MA, USA).
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