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Gold 3 chloride hydrate

Manufactured by Merck Group
Sourced in United States, France, China, Macao, Germany, Canada, Italy

Gold (III) chloride hydrate is a chemical compound composed of gold and chloride ions, with the formula AuCl3·xH2O. It is a yellow to orange-brown crystalline solid that is soluble in water and organic solvents. Gold (III) chloride hydrate is commonly used as a precursor in the synthesis of other gold compounds and as a reagent in various chemical applications.

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89 protocols using gold 3 chloride hydrate

1

Synthesis and Characterization of Carbohydrate and Aromatic Derivatives

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D-Mannose (C6H12O6; ≥99%), D-Galactose (C6H12O6; ≥99%), D-Sucrose (C12H22O11; ≥99.5%), D-Lactose monohydrate (C12H22O11·H2O; ≥99.5%), D-Cellobiose (C12H22O11; ≥99%), p-aminobenzoic acid (H2NC6H4CO2H; ≥99%), 4-aminophenyl ether (O(C6H4NH2)2; ≥97%), 4,4′-diaminobibenzyl (C14H16N2; ≥95%), 5-aminosalicylic acid (H2NC6H3-2-(OH)CO2H; ≥99%), 3-aminophenol (H2NC6H4OH; ≥98%), Penicillamine ((CH3)2C(SH)CH(NH2)CO2H; 99%), silver nitrate (AgNO3; ≥99%), and Gold (III) chloride hydrate (HAuCl4·xH2O; ≥99.995%) were purchased from Sigma–Aldrich (Ankara, Turkey). Glacial acetic acid, anhydrous acetone, anhydrous ethanol, anhydrous methanol and sodium chloride, and potassium perchlorate were purchased from Merck (Ankara, Turkey). All the chemicals were used as they were received from the manufacturer, without further purification. Deionized water (DI) (18.2 MΩ) was produced using a Zeneer purification system (Seoul, Korea).
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2

Synthesis of Gold Nanoparticles

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Silver nitrate (99%), gold (III) chloride trihydrate (HAuCl4), sodium citrate tribasic dehydrate (98%), H2O2 (30 wt%), sodium borohydride (99%), gold (III) chloride hydrate (99%), sodium hydroxide (99%), sodium sulfite (98%), ascorbic acid, poly(vinylpyrrolidone) (Mw 10, 29 and 40 kDa), anhydrous ethanol and N,N-dimethylformamide (DMF) were purchased from Sigma Aldrich (France). All aqueous solutions were prepared using purified milliQ water.
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3

Neuroprotective Potential of Hericium erinaceus

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Fresh fruiting bodies of H. erinaceus KLU-M 1232 basidiocarps were collected from Ganofarm Sdn Bhd, Tanjung Sepat, Selangor, Malaysia. Rat pheochromocytoma (PC-12) cell line was purchased from American Type Culture Collection (ATCC; Rockville, MD, USA; Catalog Number: CRL-1721.1TM). Permission to use this cell line was obtained from the Department of Veterinary Services, Ministry of Agriculture and Agro-Based Products, Malaysia. Gold (III) chloride hydrate, F-12 K medium (Kaighn’s Modification of Ham’s F-12 Medium), and NGF-7 S from murine submaxillary gland were obtained from Sigma Co. (St Louis, MO, USA). Fetal bovine serum (FBS) and horse serum (HS) were purchased from PAA Laboratories (Cölbe, Germany), and all other chemicals used for the studies were of analytical grade.
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4

Synthesis of Gold Nanoparticles

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Gold (III) chloride hydrate (HAuCl4), p-aminothiophenol (PATP), hexadecyltrimethylammonium bromide (CTAB), sodium citrate, hydroxylamine hydrochloride (NH2OH·HCl), phosphoric acid (85 wt.%), sodium nitrite (≥99.0%) were purchased from Sigma-Aldrich (Hangzhou, China). The above reagents were used without further purification and Milli-Q water (18 MΩ·cm−1) was used to prepare all aqueous solutions.
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5

Synthesis of Gold Nanoparticles in Polymer Solutions

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Gold (III) chloride hydrate, sodium hydroxide, and trisodium citrate dihydrate were purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA). Anionic, carboxylic acid modified PVA (PVA-COOH), with a viscosity of 20-30 mPa s, a degree of hydrolysis of 85-90% and a molar weight around 100000 g mol -1 , was kindly supplied by Kuraray Europe Nordic AB OY, Vantaa, Finland. Nonionic PVP-VA, with a viscosity of 26-34 mPa s and a molar weight around 65000 g mol -1 , was kindly supplied by BASF A/S, Copenhagen, Denmark. The polymers in solution were slightly acidic.
Tap water was used as a representative for groundwater, as tap water in Denmark consists of groundwater, which has undergone minimal treatments i.e. aeration and sand filtration (iron and manganese removal). The main ionic species in the tap water are bicarbonate (320 mg L -1 ), calcium (110 mg L -1 ), chloride (85 mg L -1 ), sulfate (45 mg L -1 ), sodium (35 mg L -1 ), magnesium (15 mg L -1 ), potassium (5 mg L -1 ) and nitrate (1 mg L -1 ). The tap water has a pH of 7.7. All experiments were run at room temperature.
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6

Synthesis of Microporous Metal-Organic Frameworks

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Materials.
Hexadecyltrimethylammonium bromide (CTAB) and Hexadecyltrimethylammonium chloride (CTAC) were purchased from Aladdin; HCl were purchased from Adamas; 2-methylimidazole (HMIM), Zinc nitrate hexahydrate (Zn(NO 3 ) 2 •6H 2 O), Gold(III) chloride hydrate, Tris(hydroxymethyl)aminomethane (TRIS), silver nitrate and NaCl were purchased from Sigma-Aldrich.
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7

Synthesis and Characterization of Graphene Oxide-Based Biosensors

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Gold(III) chloride hydrate, N'N-dimethylformamide (DMF), hydrazine monohydrate (NH2NH2·H2O), zirconium(IV) chloride (ZrCl4), sodium borohydride (NaBH4), and benzoic acid were obtained from Sigma-Aldrich. TCPP was purchased from Fisher Scientific. GO was prepared and characterized according to our previous work.51 (link) Commercial ELISA kits for human PSA were obtained from Abnova Corporation. All DNA strands listed in Table S1 (Supporting Information) were synthesized and purified by Integrated DNA Technologies (Coralville, IA, USA). All chemicals were of analytical grade and were used without further purification. Milli-Q water was utilized throughout this work.
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8

Synthesis of Gold Nanoparticles

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Gold nanoparticles were obtained from Nanoparticles Innovation NPIN s.c. (Łódź, Poland) . According to the manufacturer's information, AuNPs were produced by the seeded-mediated growth method (Ranoszek-Soliwoda et al. 2020) (link). Briefly, an aqueous solution of gold (III) chloride hydrate (94.25 g, 2.01•10 -4 % wt; Sigma-Aldrich, ≥ 49%) was heated to the boiling point under reflux and, next, an aqueous solution of sodium citrate (5.75 g, 0.877% wt; Sigma-Aldrich, ≥ 99.0%) was added to the reaction mixture. The solution was heated for 15 min under stirring, and then the colloid was cooled down to room temperature. The concentration of AuNPs in the resulting colloid was 100 ppm. The size and size distribution were measured by Scanning Transmission Electron Microscopy (STEM) (Nova NanoSEM 450, FEI™, Hillsboro, OR, USA), at accelerating voltage 30 kV, and reached 13 ± 3 nm (Fig. 1).
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9

Calreticulin Protein Quantification and Cell Proliferation

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Calreticulin human recombinant protein was bought from Raybiotech, Inc. Norcross, GA, (United States). Anti-proliferating cell nuclear antigen (PCNA) antibody and anti-mouse IgG2a Fluorescein IsoTioCyanate (FITC) were bought from Santa Cruz Biotechnology, Dallas, TX, United States. Calreticulin BioAssayTM Enzyme-Linked ImmunoSorbent Assay (ELISA) kit (Human) was used. Chitosan medium molecular weight (degree of deacetylation 75–85%), gold (III) chloride hydrate, dimethyl sulfoxide (DMSO), 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI), phosphate buffer saline (PBS) and antibiotic-antimicotic solution were purchased from Sigma Aldrich, St. Louis, MO, United States. Thiazolyl blue tetrazolium bromide (MTT) was purchased from Affymetrix, Inc. Cleveland, OH, United States. Fetal bovine serum (FBS), Trypsin-EDTA (1x), and Dulbecco´s Modified Eagle medium (DMEM) were purchase from Gibco, Thermo Fisher Scientific, Waltham, MA, United States. De-ionized (DI) water (Milli-Q®, Water Purification System, Merck Millipore) was also used. Human keratinocyte cells (HaCaT), human umbilical vein endothelial cells (HUVEC) and mouse fibroblast cells (NIH/3T3) were purchased from ATCC, Manassas, VA, United States.
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10

Gold (III) Chloride Hydrate Protocol

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Gold (III) chloride hydrate purchased from Sigma-Aldrich, USA; trisodium citrate purchased from EL Gomhorya Company, Egypt; deionized water; and a kit for the analysis of serum testosterone purchased from EQUIPAR Diagnostic, Serono, Italy, were used in the current study.
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