Sera were obtained from patients with established RA presenting at the Department of Medicine 3 of the University Clinic of Erlangen and of age- and sex-matched healthy volunteers. Disease activity was evaluated using the DAS28 score. Concentrations of total serum IgA, IgA1, and IgA2 was determined by ELISA using goat F(ab)2 anti-human IgA (#2052-01) as capture and HRP-coupled goat anti-IgA (#2050-05), mouse anti-human IgA1 (#9130-05), or mouse anti-human IgA2 (#9140-05) as detection antibodies (all Southern Biotech). ACPA levels were determined using cyclic-citrullinated peptide-coated plates (Orgentec Diagnostika).
Goat f ab 2 anti human iga
Manufactured by Southern Biotech
Goat F(ab)2 anti-human IgA is a laboratory reagent used for the detection and quantification of human IgA antibodies. It is a purified preparation of the F(ab)2 fragment of goat-derived polyclonal antibodies specific to the Fc region of human IgA immunoglobulins.
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Lab products found in correlation
Mouse anti human iga1, by Southern Biotech (1 mentions)
Mouse anti human iga2, by Southern Biotech (1 mentions)
Goat f ab 2 anti human igg h l, by Jackson ImmunoResearch (1 mentions)
Goat anti human igg hrp antibody, by Southern Biotech (1 mentions)
Nunc maxisorp, by Thermo Fisher Scientific (1 mentions)
2 protocols using goat f ab 2 anti human iga
1
Serum IgA Subclass Levels in Rheumatoid Arthritis
2
Quantification of Serum IgA Subclasses
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Total serum IgA1 and IgA2 levels were measured by in-house ELISA as previously described.11 (link) Briefly, ThermoFisher Nunc MaxiSorp flat-bottom plates were precoated with 2 µg/mL of goat F(ab)2 anti-human IgG (H+L) (Jackson ImmunoResearch #109-006-003) or goat F(ab)2 anti-human IgA (SouthernBiotech, #2052-01) overnight at 4°C. On the next day, plates were washed with 0.05% PBS/Tween and blocked with 1% PBS/bovine serum albumin (BSA). Sera (diluted 1:1000 000 for IgG and 1:40 000 for IgA measurement) and IgG commercial standard (Human IgG I2511 Sigma-Aldrich) or isolated IgA in known concentrations were incubated on the plates for 1 hour. After the plates were washed, they were incubated with goat anti-human IgG HRP-antibody (SouthernBiotech #2040-05) for 1 hour. After detection with TMB substrate, the absorption was measured at 450 nm with reference wavelength at 650 nm.
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