Ab11575

Manufactured by R&D Systems

Sourced in United States

Ab11575 is an antibody reagent produced by R&D Systems. It is a primary antibody used for the detection of its target protein in various immunoassay applications.

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Lab products found in correlation

Senescence detection kit, by Abcam (1 mentions) Ab34710, by Abcam (1 mentions) Matrigel, by Abcam (1 mentions) Ab1827, by Abcam (1 mentions) Ab5539, by Merck Group (1 mentions) Cyanine 3, by Jackson ImmunoResearch (1 mentions) Af3076, by R&D Systems (1 mentions) Alexa 488, by Jackson ImmunoResearch (1 mentions)

2 protocols using ab11575

Extracellular Matrix Regulation Assay

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All the reagents used in this study were supplied by ThermoScientific USA (Waltham, MA, USA) unless otherwise stated. Antibodies against TIMP-1 (Abcam: Ab1827) (Cambridge, MA, USA), MT1-MMP (Abcam: Ab38970), fibronectin (R&D Systems: MAB 19181) (Minneapolis, MN, USA), collagen I (Abcam: Ab34710) and laminin (Abcam: Ab11575) were purchased either from R&D Systems or Abcam, USA. Senescence detection kit and Matrigel^® were products from Biovision (San Francisco, CA, USA) and BD Biosciences (San Jose, CA, USA). HT1080 and CaKi-1 cell lines were acquired from the Shanghai Cell Repository, Chinese Academy of Science (Shanghai, China).

Jiang B., Liu J, & Lee M.H. (2019). Targeting a Designer TIMP-1 to the Cell Surface for Effective MT1-MMP Inhibition: A Potential Role for the Prion Protein in Renal Carcinoma Therapy. Molecules, 24(2), 255.

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Immunostaining of Mouse Embryonic Spinal Cords

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Mouse embryonic spinal cords (E10.5-E12.5) were fixed in 4% paraformaldehyde for 2 hours at 4°C, cryoprotected in 30% sucrose in PBS overnight and thin-sectioned to yield 30μm transverse sections. Antibody staining was performed by incubating the sections with primary antibodies at 4°C overnight, followed by fluorescently-labeled secondary antibodies at room temperature for 2 hours. Antibodies against the following proteins were used for immunostaining: Rabbit: neurofilament (NF), 1:200 (Cell Signaling Technology C28E10); Shh, 1:200 (H4 (Ericson et al., 1996)); Laminin, 1:1000 (Abcam #ab11575); Goat: human Robo3, 1:200 (R&D Systems AF3076); mouse Dcc, 1:500 (R&D Systems AF844); mouse netrin1, 1:500 (R&D Systems AF1109); β-galactosidase, 1:2000 (Biogenesis 4600-1409); Sox2, 1:2000 (Santa Cruz Biotechnology #17320); Mouse: cre, 1:1000 (Covance MMS-106P); Sox2, 1:1000 (Santa Cruz Biotechnology #365823); mAb Tag1 1:100 (4D7, Developmental Studies Hybridoma Bank (DSHB)); NF, 1:100 (3A10 DSHB); Nestin, 1:50 (Rat-401 DSHB); Chicken: GFP, 1:1000 (Aves Lab #1020); neurofilament, 1:2000 (Millipore #AB5539). Secondary antibodies (all from Jackson Immunoresearch Laboratories) were used as follows: FITC, 1:500; Alexa488, 1:1000; Cyanine3, 1:1000; Cyanine5 1:700.

Varadarajan S.G., Kong J.H., Phan K.D., Kao T.J., Panaitof S.C., Cardin J., Eltzschig H., Kania A., Novitch B.G, & Butler S.J. (2017). Netrin1 produced by neural progenitors, not floor plate cells, is required for axon guidance in the spinal cord. Neuron, 94(4), 790-799.e3.

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