Log In Sign up
The largest database of trusted experimental protocols

Automated platform

Manufactured by Agilent Technologies
Visit Supplier

The Automated platform is a versatile lab equipment designed to streamline various laboratory processes. It features a modular and customizable design, enabling users to configure the system to meet their specific needs. The core function of the Automated platform is to automate repetitive tasks, improve efficiency, and enhance the consistency of experimental outcomes.

Automatically generated - may contain errors

Lab products found in correlation

Decloaker, by Biocare Medical (1 mentions) Clone sp263, by Roche (1 mentions)

Close spelling variants of this product

Fragment Analyzer Automated CE platform Dako Automated Link 48 platform Bravo Automated Liquid Handling Platform Automated Link 48 platform Agilent Bravo Automated Liquid Handling Platform Bravo automated platform Omnis automated staining platform

3 protocols using automated platform

1

Immunofluorescence Analysis of Cardiac Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three WT and 3 Des−/− hearts from 6-mo-old male mice were embedded in paraffin and sectioned for immunofluorescence analysis. The care and treatment of animals used in the study followed the institutional approved guidelines. Formalin-fixed, paraffin-embedded sections of normal canine heart and tongue were evaluated using immunofluorescence detection. Paraffin-embedded multitissue sections (∼ 5 μm) were deparaffinized in xylene and rehydrated with graded alcohols. Heat-induced antigen retrieval was performed in a pressure cooker (Decloaker, Biocare Medical, Concord, CA) using citrate buffer at pH 6. The immunohistochemical procedure was performed using an automated platform (Dako, Carpinteria, CA). The total intensity of nebulette and desmin in stained-tissue images was quantified to determine the intensity value/pixel, and cumulative histogram distributions were generated using a LaTeX algorithm written by L. Macri (Texas A&M University, College Station, TX. The distance between nebulette striations was analyzed using the line tool in heart tissue sections stained for nebulette to generate line profiles using ImageJ (National Institutes of Health).
Hernandez D.A., Bennett C.M., Dunina-Barkovskaya L., Wedig T., Capetanaki Y., Herrmann H, & Conover G.M. (2016). Nebulette is a powerful cytolinker organizing desmin and actin in mouse hearts. Molecular Biology of the Cell, 27(24), 3869-3882.
+ Open protocol
+ Expand
2

Breast Cancer Epidemiology in Kenya

Check if the same lab product or an alternative is used in the 5 most similar protocols
Relevant socio-demographic, reproductive, and clinical data were collected from all consenting patients using structured questionnaires and clinical data abstraction case report forms.
The ethnicity of patients was determined through self-reporting by respondents of their parents’ and maternal and paternal grandparents’ tribal affiliations. The tribes were assigned to the corresponding three major ethnic groups; Bantu, Nilotes and Cushites[9 ] If all maternal and paternal grandparents and parents did not belong to the same tribe, that patient’s ethnicity was categorized as mixed. Due to sparse numbers, patients reporting “mixed” ethnicity (N=23, 2.7%) were excluded from the analytic population.
All breast cancer tissues blocks were submitted to Aga Khan University Hospital, Nairobi to undergo central pathology review and immunohistochemistry. Tumor size, tumor grade, presence of lympho-vascular invasion, lymph node metastases and extra nodal extension were documented. ER/PR/HER2 status was analyzed on the Dako Automated platform as previously reported,[10 ] and tumors were assigned into 3 major breast cancer molecular subtypes based on immunohistochemistry: ER and/or PR positive and HER2 positive or negative (Luminal A/B), ER/PR negative and HER2 positive (HER2 enriched), and ER/PR and HER2 negative (Triple Negative).
Sayed S., Moloo Z., Wasike R., Bird P., Oigara R., Njoroge F.W., Jamal A., Prasad S.V., Vinayak S., Gierach G.L., Dawsey S.M., Palakal M., Fan S., Mullooly M., Chauhan R., Okiro P., Gakinya S., Nzioka A., Kyobutungi C., Mohamed S., Haregu T., Mussajee M., Bonass B., Mariwa C., Sherman O.A., Mohammed A., Gachii A., Githaiga J., Karanu J., Nyagah R., Njoroge R., Muramba I., Otieno J.O., Raburu D.O., Mwachiro E.B., Abayo I, & Saleh M. (2017). ETHNICITY AND BREAST CANCER CHARACTERISTICS IN KENYA. Breast cancer research and treatment, 167(2), 425-437.
+ Open protocol
+ Expand
3

Immunohistochemistry Analysis of PD-L1 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three-micrometer-thick sequential histologic tumor sections were obtained from representative formalin-fixed paraffin-embedded tumor blocks (whole-face or TMA) and used for IHC analysis. IHC was performed using an automated staining system (Ventana BenchMark) with antibodies against PD-L1 (SP263 clone, Ventana, CC1 pre-treatment for 64 minutes, Ventana Optiview detection protocol) or using a Dako automated platform with antibody to the 22C3 clone of PD-L1. Both systems used a diaminobenzidine reaction to detect antibody labeling and hematoxylin counterstaining.
Humphries M.P., McQuaid S., Craig S.G., Bingham V., Maxwell P., Maurya M., McLean F., Sampson J., Higgins P., Greene C., James J, & Salto-Tellez M. (2019). Critical Appraisal of Programmed Death Ligand 1 Reflex Diagnostic Testing: Current Standards and Future Opportunities. Journal of Thoracic Oncology, 14(1), 45-53.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!