Recombinant human amphiregulin

A rabbit polyclonal anti-actin antibody, EGFR inhibitor (AG1478) and 2-deoxy-D-glucose (2-DG) were obtained from Sigma-Aldrich (St. Louis, Mo., USA). Dobutamine hydrochloride was obtained from Wako Laboratory Chemicals (Tokyo, Japan). Recombinant human amphiregulin was obtained from PeproTech (Rocky Hill, NJ). Rabbit polyclonal anti-lipolysis-stimulated receptor (LSR) and anti-tricellulin (TRIC) antibodies were obtained from Zymed Laboratories (San Francisco, CA). A mouse monoclonal anti-LSR antibody was obtained from Abnova (Yaipei, Taiwan). A rabbit polyclonal anti-angiomotin (AMOT) antibody was obtained from Bioss, Inc. (Woburn, MA). Rabbit monoclonal anti-Merlin, anti-phospho- YAP (ser127) and anti-phospho-AMPK (ther172) antibodies were obtained from Cell Signaling Technology Japan (Tokyo, Japan). A rabbit polyclonal anti-YAP antibody was obtained from Novus Biologicals (Littleton, CO). Alexa 488 (green)-conjugated anti-rabbit IgG and Alexa 594 (red)-conjugated anti-mouse IgG antibodies were purchased from Molecular Probes, Inc. (Eugene, OR).

All chemicals were purchased from Sigma-Aldrich (MO, USA), unless specified otherwise. LH was purchased from Sansheng Biological Technology (Ningbo, China). Recombinant human amphiregulin, recombinant human epiregulin and recombinant human betacellulin were purchased from PeproTech (NJ, USA). AG1478 was purchased from Abmole Bioscience (TX, USA). Cycloheximide was purchased from Apexbio Technology (TX, USA).