Blood samples were collected at three time points from the cubital vein: before testing (pre-test); no longer than 5–15 min after exercise (post-test); and about 1 h later, at the end of the lactate recovery period (LA-rec; Faude et al., 2009 (link); Beneke et al., 2011 (link); Smekal et al., 2012 (link)). At each time point, venous blood samples were collected in a 7.5 ml S-Monovette tube for serum separation (SARSTEDT AG & Co., Nümbrecht, Germany) and a 7.5 ml S-Monovette tube with ethylenediaminetetraacetic acid (EDTA K3, 1.6 mg EDTA/ml blood) for immune cell analyses (SARSTEDT). All analyses were performed immediately following blood collection and serum separation except for evaluation of phosphorylation of selected proteins.
S monovette tube
Manufactured by Sarstedt
Sourced in Germany, Austria, Australia
The S-Monovette tubes are a collection of blood collection devices manufactured by Sarstedt. They are designed for the safe and efficient collection of blood samples for laboratory analysis.
Automatically generated - may contain errors
Lab products found in correlation
K3 edta, by Sarstedt (4 mentions)
Edta ke, by Sarstedt (2 mentions)
Mirneasy serum plasma kit, by Qiagen (2 mentions)
Clotting activator, by Sarstedt (2 mentions)
Cobas 6000 analyzer, by Roche (2 mentions)
S monovette, by Sarstedt (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Separating gel, by Sarstedt (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Sarstedt (1 mentions)
Fetal bovine serum (fbs), by PAN Biotech (1 mentions)
Amphotericin b, by Thermo Fisher Scientific (1 mentions)
Luminex assay, by DiaSorin (1 mentions)
S monovette collection tubes, by Sarstedt (1 mentions)
Bcs xp analyzer, by Siemens (1 mentions)
Nexera x2 uhplc, by Shimadzu (1 mentions)
8050 triple quadruple detector, by Shimadzu (1 mentions)
Clot activator, by Sarstedt (1 mentions)
Nunc cryotube, by Thermo Fisher Scientific (1 mentions)
Bio plex pro rbm human metabolic panel 2, by Bio-Rad (1 mentions)
61 protocols using s monovette tube
1
Blood Sampling for Exercise Physiology
2
Blood Sample Collection and Processing
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Fasting blood samples were obtained in the morning from the elbow vein. Blood samples from each participant were taken into two tubes. For biochemical analyses, a 4.9 mL S-Monovette tube with ethylenediaminetetraacetic acid (K3EDTA; 1.6 mg EDTA/mL blood) and separating gel (SARSTEDT AG & Co., Nümbrecht, Germany) was used. For complete blood count, a 2.6 mL S-Monovette tube with K3EDTA (1.6 mg EDTA/mL blood) (SARSTEDT AG & Co., Nümbrecht, Germany) was used. Blood samples for biochemical analyses were centrifuged 300 × g for 15 minutes at room temperature in order to receive blood plasma.
3
Blood Sampling Procedure for Exercise Physiology
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Blood samples were collected at three time points from the cubital vein, namely before testing (pre-test), no longer than 5–15 min after exercise (post-test), and approximately 1 h later, at the end of the lactate recovery period [35 (link),36 (link),37 (link)]. At each time point, venous blood samples were collected in a 7.5 mL S-Monovette tube for serum separation (SARSTEDT AG & Co., Nümbrecht, Germany) and a 7.5 mL S-Monovette tube with ethylenediaminetetraacetic acid (EDTA K3, 1.6 mg EDTA/mL blood) for immune cell analyses (SARSTEDT). All analyses were performed immediately following blood collection and serum separation except for the evaluation of lactate concentration.
It is known that exercise may cause changes in the plasma volume and therefore changes in analyzed cell counts. Therefore, to compensate for this phenomenon, plasma volume loss (ΔPV) was calculated according to the classic equations from Dill and Costill, provided by Alis et al. [38 (link)]:
where: Hbpre = hemoglobin pre-test (g/dL); Hbpost = hemoglobin post-test (or in recovery; g/dL); Htcpre = hematocrit pre-test (%); and Htcpost = hematocrit post-test (or in recovery; %).
Then, the corrected blood parameter values were calculated using the formula:
It is known that exercise may cause changes in the plasma volume and therefore changes in analyzed cell counts. Therefore, to compensate for this phenomenon, plasma volume loss (ΔPV) was calculated according to the classic equations from Dill and Costill, provided by Alis et al. [38 (link)]:
where: Hbpre = hemoglobin pre-test (g/dL); Hbpost = hemoglobin post-test (or in recovery; g/dL); Htcpre = hematocrit pre-test (%); and Htcpost = hematocrit post-test (or in recovery; %).
Then, the corrected blood parameter values were calculated using the formula:
4
Peripheral Blood Sample Collection and PBMC Isolation for Myasthenia Gravis Research
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Peripheral blood samples were collected from 12 healthy controls, six female and six male participants with an average age of 42, and 17 patients with clinically confirmed myasthenia gravis showing AChR-autoantibody RIA measurements above 0.5 nmol/l, with 6 female and 12 male participants and an average age of 62 (Supplementary Table 1).
Peripheral blood was drawn into S-Monovette tubes containing 1.6 mg EDTA per ml blood (01.1605.100) for isolation of PBMC, and into S-Monovette tubes with clot activator (01.1601.100, both from Sarstedt) for serum preparation. PBMC isolation and serum preparation were performed as previously described [1 (link), 47 (link)]. PBMC were stored in liquid nitrogen until use, serum was stored at − 20 °C. The project was reviewed and authorized by the Ethikkommission Nordwest und Zentralschweiz.
Peripheral blood was drawn into S-Monovette tubes containing 1.6 mg EDTA per ml blood (01.1605.100) for isolation of PBMC, and into S-Monovette tubes with clot activator (01.1601.100, both from Sarstedt) for serum preparation. PBMC isolation and serum preparation were performed as previously described [1 (link), 47 (link)]. PBMC were stored in liquid nitrogen until use, serum was stored at − 20 °C. The project was reviewed and authorized by the Ethikkommission Nordwest und Zentralschweiz.
5
Blood Sampling and Analysis Protocol
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Blood samples (5 mL of venous blood) were collected from each patient once after a 12-hour fasting in the morning (between 07.00 and 09.00 a.m., limited physical activity) into a S-Monovette tube with a coagulation activator (Sarstedt AG & Co., Numbrecht, Germany). Radiographs were also completed at the same time on the same day. The sera for biochemical assays were separated by centrifugation at 1500xg for 10 minutes, frozen and stored at - 86 ºC until analysis. Besides serum, a portion of each blood sample was collected into the S-Sedivette tube (Sarstedt AG & Co., Numbrecht, Germany) containing anticoagulant liquid sodium citrate for determination of erythrocyte sedimentation rate and into the S-Monovette K2 EDTA tube (Sarstedt AG & Co., Numbrecht, Germany) for haematological analysis.
6
Piglet Castration NSAID Cortisol
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Blood samples were taken after randomization of the piglets, directly before the application of the NSAID, as well as 5 min. and 120 min. after castration by puncture of the Vena cava cranialis. One ml of blood was collected into a S-Monovette tube (Sarstedt, Germany). Samples were stored at 4 °C, serum was gained by standard centrifugation and frozen at -18 °C until further usage. Plasma cortisol concentrations were determined in ng/ml using an IMMULITE system (Siemens Diagnostic, USA). The intra-assay coefficient of variation was 6.3–10.0 % and the inter-assay coefficient of variation was 5.8–8.8 %.
7
Renalase Serum-Urine Balance Protocol
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Blood and urine were collected in the morning on empty stomach, in the shortest possible time interval to give the most accurate information about momentary serum-to-urine renalase balance. Whole blood was drawn into S-Monovette tube (Sarstedt, Numbrecht, Germany) with a clotting activator and left for 30 min at room temperature. Serum was obtained by centrifugation of the clotted blood (10 min, 1000 x g, room temperature). Urine was collected into dedicated sterile urine containers and centrifuged for 20 min (600 x g, room temperature). Both materials were then transferred to smaller tubes and kept in the refrigerator at − 80 °C until use. Since this study did not involve 24-h urine collection, the concentration of urinary renalase was also normalized by comparison to urinary creatinine.
8
Exercise-Induced Blood Sampling Protocol
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Blood samples were obtained tree times from the elbow vein: before the testing (pre-exercise), no longer than 5 minutes after the test (post-exercise) and ca. 17 hours after the test, at the end of recovery time (recovery). Each time, blood samples were taken into 7.5 mL S-Monovette tube with ethylenediaminetetraacetic acid (EDTA K3, 1.6 mg EDTA/mL blood) (SARSTEDT AG & Co., Nümbrecht, Germany). All analyses were performed immediately after the blood collection.
Importantly, for safety of the participants, the test protocol requires them to be after a light breakfast. Therefore, the blood samples collected after the test, with the exception for recovery time, were not fasting blood.
Importantly, for safety of the participants, the test protocol requires them to be after a light breakfast. Therefore, the blood samples collected after the test, with the exception for recovery time, were not fasting blood.
9
Blood Sample Collection and PBMC Isolation
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Blood samples were collected from each participant by venipuncture in a 7,5 ml S-Monovette tube with clot activator (Sarstedt, Germany) and 4,9 ml S-Monovette tube with EDTA (Sarstedt, Germany) on hospital admission before treatment administration and on the fifth day of treatment. The collected samples were centrifuged, and serum and plasma were frozen at -80 degrees Celsius. Density gradient centrifugation with Pancoll 1.077 g/l (PAN Biotech, Germany) was used for isolation of PBMCs, then the cells were washed twice in phosphatate-buffered saline (Corning, VA, USA) and cryopreserved in fetal bovine serum (PAN Biotech, Germany) with 10% dimethyl sulfoxide (Sigma-Aldrich, MO, USA).
10
Venous Blood Sampling for Lactate Recovery
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Venous blood for the analyses were sampled three times: before the test (pre-test), immediately after the test (not longer than 5 min, post-test) and after lactate recovery (60 min after the test, LA-rec) [42 (link),43 (link)]. Peripheral blood samples were collected from the cubital vein. Additionally, to confirm lactate recovery, blood levels of lactate acid were determined. Venous blood samples were collected in a 7.5 mL S-Monovette tube with ethylenediaminetetraacetic acid (EDTA K3, 1.6 mg EDTA/mL blood) (SARSTEDT AG & Co., Nümbrecht, Germany).
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