Fitc labeled anti mcd11c

On day 28 after establishment of the subcutaneous tumor model, splenocytes or blood was isolated from each group. After lysing red blood cells, the remaining cells were stained with FITC-labeled anti-mCD11c (catalog number: 11-0114), PE-labeled anti-mCD80(catalog number: 12-0801), FITC-labeled anti-mCD4 (catalog number: 11-0042), or APC-labeled anti-mCD8 (catalog number: 17-0081) (all from eBioscience) for 30 minutes at room temperature. The positive cells were measured by flow cytometry.

Tumor tissues were obtained from 20 PCa patients and normal tissues were selected from 10 patients who received pathological biopsy of benign disease in Nanfang Hospital (Guangzhou, China). None of these patients had received immune‐regulating drugs or radiation therapy before surgery. The study was approved by the ethics committee and informed consent was obtained from all patients. Norcantharidin, CCK‐8, and DAPI reagents were purchased from Sigma (St. Louis, MO, USA). Streptavidin‐tagged mouse GM‐CSF (SA‐mGM‐CSF) fusion protein was prepared by our laboratory. Western blotting antibodies against p‐ERK1/2, p‐Akt, ERK1/2, Akt, Bcl‐2, FOXO1, FasL, and GAPDH were purchased from Abcam (Cambridge, UK). Phycoerythrin‐labeled anti‐mGM‐CSF, FITC‐labeled anti‐mCD11c, PE‐labeled anti‐mCD80, FITC‐labeled anti‐mCD4, FITC‐labeled anti‐mCD8 and PerCP‐Cyanine 5.5‐labeled anti‐mFoxP3 antibodies were purchased from eBioscience (San Diego, CA, USA). C57BL/6 male mice (6‐8 weeks old) were purchased from the experimental animal center of Southern Medical University (Guangzhou, China). All animal studies were undertaken in accordance with the Southern Medical University guidelines for experimental animals.