5 ht creatinine sulfate

Manufactured by Merck Group

Sourced in United States

5-HT creatinine sulfate is a chemical compound used in laboratory research. It is a salt formed by the combination of 5-hydroxytryptamine (serotonin) and creatinine sulfate. This product is commonly used as a research tool in the study of serotonin signaling and related biological processes.

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7 protocols using 5 ht creatinine sulfate

Neurotransmitter Receptor Ligands Assay

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Acetylcholine chloride, 5-CT maleate, 5-HT creatinine sulfate, forskolin, and norepinephrine hydrochloride were obtained from Sigma Chemical Company (St. Louis, MO). 8-OH-DPAT, AS-19, BW723C86, CP93129, LP-44, LY215840, LY272015, mCPBG, PNU109291, SB269970, and TCB-2 were purchased from Tocris (R& D systems, Minneapolis, MN). ET-1 (1-21) was purchased from Bachem (Torrance, CA).

Watts S.W., Darios E.S., Seitz B.M, & Thompson J.M. (2015). 5-HT is a potent relaxant in rat superior mesenteric veins. Pharmacology Research & Perspectives, 3(1), e00103.

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Bupropion and Paroxetine Binding Assay

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Bupropion and paroxetine (Sequoia Research Products, Pangbourne, UK). WAY 100635, 5-HT creatinine sulfate, and chloral hydrate were purchased from Sigma (St. Louis, MO). Quisqualic acid was purchased from Tocris (Ellisville, MO).

Mansari M.E., Manta S., Oosterhof C., El Iskandrani K.S., Chenu F., Shim S, & Blier P. (2015). Restoration of Serotonin Neuronal Firing Following Long-Term Administration of Bupropion but Not Paroxetine in Olfactory Bulbectomized Rats. International Journal of Neuropsychopharmacology, 18(4), pyu050.

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Vortioxetine and Escitalopram Pharmacology

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Vortioxetine and escitalopram were provided by Lundbeck. WAY-100635, 5-HT creatinine sulfate, and chloral hydrate were purchased from Sigma (St. Louis, MO, USA). Quisqualic acid and CP 94253 were purchased from Tocris (Ellisville, MO, USA). Vortioxetine and CP 94253 were dissolved in 20% hydroxypropyl-beta-cyclodextrin. Escitalopram was dissolved in a 0.9% NaCl solution. WAY-100635 was dissolved in distilled water.

El Mansari M., Lecours M, & Blier P. (2015). Effects of acute and sustained administration of vortioxetine on the serotonin system in the hippocampus: electrophysiological studies in the rat brain. Psychopharmacology, 232(13), 2343-2352.

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Serotonin Regulates Shrimp Ovarian Maturation

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Neurotransmitters like serotonin (5-HT) is involved in the regulation of ovarian maturation and ovulation. To examine effects of 5-HT on PmE2F-2 mRNA expression, 5-HT creatinine sulfate (Sigma, MO, USA) was dissolved in the sterilized saline solution and made into a 0.25 μmol solution. The first abdominal segment of female shrimp was injected intramuscularly with 50 μL 0.25 μmol 5-HT. Other shrimps were injected with sterilized saline solution (10 mM Tris—HCl at pH 7.5, 400 mM NaCl) at 0 h and were used as control. Ovaries were collected at 0, 6, 12, 24, 48, 72, and 96 h postinjection, snap frozen in liquid nitrogen, and stored at −80°C.
After unilateral eyestalk ablation, ovaries of female shrimp were collected at 0, 3, 6, 12, 24, 48, 72, and 96 h, snap frozen in liquid nitrogen, and stored at −80°C to analyze effects of the process on PmE2F-2 mRNA expression.

Xie B., Wang P., Zhao C, & Qiu L. (2017). Genomic structure, expression pattern, and functional characterization of transcription factor E2F-2 from black tiger shrimp (Penaeus monodon). PLoS ONE, 12(5), e0177420.

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Pharmacological Manipulation of 5-HT Signaling

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5-HT creatinine sulfate (Sigma-Aldrich, St. Louis, MO, USA) was diluted in 0.1% ascorbic acid in saline and administered iv (8.3 εg/min; 500 εg/ml at an infusion rate of 1 ml/h) ^{68 (link),69 (link)}, or by nanoinjection in the rRPa (33 mM, 60 nl). 8-OH-DPAT (Sigma-Aldrich, St. Louis, MO, USA) was diluted in saline and administered iv (100 εg, 500 εl) or in the rRPa (10 mM, 60 nl). WAY100635 (10 mM, 100 nl), N-methyl-d-aspartate (NMDA, 0.2 mM, 60 nl), bicuculline (500 εM, 100 nl), and muscimol (2 mM, 80 nl), were purchased from Tocris (Bristol, UK) and diluted in saline.

Mota C.M., Branco L.G., Morrison S.F, & Madden C.J. (2019). Systemic serotonin inhibits brown adipose tissue sympathetic nerve activity via a GABA input to the dorsomedial hypothalamus, not via 5HT1A receptor activation in raphe pallidus. Acta physiologica (Oxford, England), 228(3), e13401.

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Neurotransmitter Receptor Pharmacology

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Materials were purchased from US companies and solubilized in the carrier noted in parentheses. 5-carboxamidotryptamine maleate (water), 5-HT creatinine sulfate (water), ascorbate (water), gallamine, hexamethonium, ketanserin (DMSO, used dilutions in water), noradrenaline hydrochloride (water), phenylephrine hydrochloride (water) and urethane were obtained from Sigma Chemical Company (St. Louis, MO, USA). 8-OH-DPAT (water), CP93129 (water), TTX (water) and UK14304 (dimethylsulfoxide) were purchased from Tocris (R&D systems, Minneapolis, MN, USA). Fatal Plus (Pentobarbital 390 mg/ml) was purchased from Vortech Pharmaceutical (Dearborn MI USA). Isoflurane (IsofloTM) was purchased from Abbott (USA) and Enrofloxacin from Bayer Healthcare LLC (USA).

Darios E.S., Barman S.M., Orer H.S., Morrison S.F., Davis R.P., Seitz B.M., Burnett R, & Watts S.W. (2015). 5-Hydroxytryptamine does not reduce sympathetic nerve activity or neuroeffector function in the splanchnic circulation. European journal of pharmacology, 754, 140-147.

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Neurotransmitter Compound Acquisition

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5-HT creatinine sulfate and norepinephrine hydrochloride were obtained from Sigma Chemical Company (St. Louis, MO USA). 5-CT, SB269970, DR4485, TCB-2, NBOH and MDL 11,939 were purchased from Tocris (R & D systems, Minneapolis, MN, USA).

Gonzalez-Pons R., McRae K., Thompson J.M, & Watts S.W. (2021). The 5-HT7 receptor restrains 5-HT-induced 5-HT2A mediated contraction in the isolated abdominal vena cava. Journal of cardiovascular pharmacology, 78(2), 319-327.

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