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2 protocols using invivomab anti mouse ly6g

1

Inflammatory Pathway Activation Assays

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InVivoMab anti-mouse Ly6G (CAT#: BE0075-1) was purchased from Bio X Cell (Lebanon, NH, USA). Compound C (CpC, CAT#: 171260), LPS (E. coli O111:B4, CAT#: L4391), STO-609 (STO, CAT#: 570250) and lucigenin (CAT#: M8010) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mouse interleukin-1β (IL-1β)/IL-1F2 Quantikine ELISA Kit (CAT#: MLB00C) and Mouse IL-6 Quantikine ELISA Kit (CAT#: M6000B) were purchased from R&D Systems, Inc. (Minneapolis, MN, Canada). MojoSort™ Human Pan Monocyte Isolation Kit (CAT#: 480060) was purchased from BioLegend (USA). Human Macrophage Colony-Stimulating Factor (M-CSF) Recombinant Protein (CAT#: RP-8643), Pierce™ BCA Protein Assay Kit (CAT#: 23227) and Amplex Red Hydrogen Peroxide⁄Peroxidase Assay Kit (CAT#: A22188) were purchased from Invitrogen (Carlsbad, CA, USA). Lactate Dehydrogenase (LDH) Assay Kit (CAT#: A020-2), Myeloperoxidase (MPO) Assay Kit (CAT#: A044-1-1), ROS Assay Kit (CAT#: E004-1-1), Malondialdehyde (MDA) Assay Kit (CAT#: A003-1-1), 4-Hydroxynonenal ELISA Kit (CAT#: H268), Protein Carbonyl Assay Kit (CAT#: A087-1-1), Total Anti-oxidant Capacity Assay Kit (CAT#: A015-2-1) and Superoxide Dismutase (SOD) Assay Kit (CAT#: A001-3-2) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). TransAM® NF-κB p65 Kit was purchased from Active Motif (Carlsbad, CA, USA).
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2

Multiparametric Flow Cytometry Analysis of Murine Immune Cell Populations

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The following flow antibodies were purchase from Biolegend: Antimouse CD45-Brilliant Violet 421; Antimouse Ly-6G-Alexa Fluor 488; Antimouse Ly-6G-Alexa Fluor 647; Antimouse CD31-Alexa Fluor 647; Antimouse CD31-PE/Cyanine7; Antimouse CD45 FITC; Anti-mouse/human CD11b-Brilliant Violet 421; Antimouse CD184 (CXCR4)-PerCP/Cyanine5.5; Antimouse CD182 (CXCR2)-APC/Cyanine7.
The following flow antibodies were purchase from Invitrogen: Anti-CD144 (VE-cadherin)-eFluor 660; Anti-CD54 (ICAM-1)-PE; Anti-CD11b-APC; Antimouse F4/80-PE.
The following flow antibodies were purchase from BD Biosciences: Antimouse Ly6G-PE; Anti-Mouse CD16/CD32. Anti-Fibrin (Cat#MABS2155) was purchased from Sigma-Aldrich; Anti-SOD2/MnSOD (Cat#ab68155) and Anti-CD15 (Cat#ab135377) were was purchased from Abcam; Antirabbit β-Actin-HRP (Cat#AF5006) was purchased from Beyotime; Antirabbit IgG-HRP (Cat#7074 S), anti-Ly6G (Cat#87048 S) and anti-COX IV (Cat#4844 S) were purchased from Cell Signaling Technology; Anti-TOMM22 (Cat#66562-1-Ig) was purchased from Proteintech; InVivoMAb antimouse Ly6G (Cat#BE0075-1) and InVivoMAb rat IgG2a isotype control (Cat#BE0089) were purchase from Bio X Cell; Donkey antirabbit IgG (H + L)-Cy3 (Cat#711-165-152) and donkey anti-mouse IgG (H + L)-Cy3 (Cat#715-165-150) were purchased from Jackson ImmunoResearch.
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