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Bc1580

Manufactured by Solarbio
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Sourced in China

The BC1580 is a laboratory centrifuge with a maximum speed of 15,000 rpm and a maximum relative centrifugal force (RCF) of 21,130 x g. It features a brushless motor and a digital display for speed and time control.

Automatically generated - may contain errors

Lab products found in correlation

Ab204718, by Abcam (1 mentions) Ab83431, by Abcam (1 mentions) Bc2505, by Solarbio (1 mentions) Bc2200, by Solarbio (1 mentions) Bc2150, by Solarbio (1 mentions)

3 protocols using bc1580

1

Metabolic Profiling Assay Protocol

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The levels of glucose, pyruvate, citric acid, and glutamic acid were determined with an assay kit (BC2505, BC2200, BC2150, BC1580, Solarbio, Beijing, China). The levels of succinate and α-ketoglutarate were determined with an assay kit (ab204718, ab83431, abcam, Cambridge, UK).
Li F., Liu H., Wu X., Song Z., Tang H., Gong M., Liu L, & Li F. (2023). Tetrathiomolybdate Decreases the Expression of Alkaline Phosphatase in Dermal Papilla Cells by Increasing Mitochondrial ROS Production. International Journal of Molecular Sciences, 24(4), 3123.
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2

Quantifying Metabolic Enzyme Activities

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The following kits were used to detect GLS1 enzyme activity, α‐KG content and glutamine content of cells and tissues respectively: the GLS1 activity test kit (BC1455, Solarbio), the α‐KG enzyme‐linked immunosorbent assay (ELISA) Kit (2M‐KMLJM220809m and 2H‐KMLJh313735, Camilo Biological, Nanjing, Jiangsu, China) and the glutamic acid (Glu) content detection kit (BC1580, Solarbio). All experimental procedures followed the instructions of the kits.
Hu X., Ma Z., Xu B., Li S., Yao Z., Liang B., Wang J., Liao W., Lin L., Wang C., Zheng S., Wu Q., Huang Q., Yu L., Wang F, & Shi M. (2023). Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2‐positive gastric cancer. Cancer Communications, 43(8), 909-937.
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3

Glutamic Acid Quantification Protocol

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The intracellular levels of glutamic were determined with an assay kit (BC1580, Solarbio, Beijing, China). Glutamic content was detected, and the absorbance was measured at 340 nm.
Li F., Wu X., Liu H., Liu M., Yue Z., Wu Z., Liu L, & Li F. (2022). Copper Depletion Strongly Enhances Ferroptosis via Mitochondrial Perturbation and Reduction in Antioxidative Mechanisms. Antioxidants, 11(11), 2084.
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