This research was approved by the Ethics Committee of The Fourth Hospital of Hebei Medical University. Five pairs of lung cancer tissues and adjacent tissues were derived from patients in the Fourth Hospital of Hebei Medical University with signed informed consent. The tissue samples were preserved at −80°C until subsequent detection. Lung cell lines A549, SK-MES-1, NCI-H1437, and NCI-H1975 were purchased from Procell Life Science&Technology Co., Ltd (Wuhan, China), and NCI-H2170 was purchased from Zhongqiaoxinzhou Biotech Co., Ltd (Shanghai, China). A549 was cultured in Ham’s F-12 K (PM150910, Procell Life Science) with 10% fetal bovine serum (FBS; SH30084.03, Hyclone, USA), while SK-MES-1 was maintained in MEM (41,500–067, Gibco, USA) with 10% FBS. NCI-H1437, NCI-H2170, and NCI-H1975 were grown in RPMI1640 (31,800–014, Gibco) with 10% FBS. All cells were cultured in an incubator (HF-90, Shanghai Lishen, China) of 95% humidity, 5% CO2 at 37°C.
Nci h1437
Manufactured by Procell
Sourced in China
NCI-H1437 is a human lung cancer cell line derived from a non-small cell lung carcinoma. It is a widely used in vitro model for studying lung cancer biology and testing potential therapeutic agents.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Sk mes 1, by Procell (1 mentions)
Nci h1975, by Thermo Fisher Scientific (1 mentions)
Sk mes 1, by Thermo Fisher Scientific (1 mentions)
Nci h1437, by Thermo Fisher Scientific (1 mentions)
Nci h2170, by Thermo Fisher Scientific (1 mentions)
Nci h1975, by Procell (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Hcc827, by Procell (1 mentions)
Ham s f 12k medium, by Procell (1 mentions)
A549 cells, by Procell (1 mentions)
Nci h1299, by Procell (1 mentions)
2 protocols using nci h1437
1
Lung Cancer Tissue and Cell Line Sourcing
2
Transfection of Circular RNA in Lung Cancer Cells
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Human bronchial epithelial cells (HBE) were obtained from FUHENG Biology (Shanghai, China). PLA-801D, NCI-H1299, HCC827, NCI-H1437 and NCI-H446 cells (Procell, Wuhan, China) were maintained in RPMI-1640 (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS) (Hyclone, Logan, UT, USA). A549 cells (Procell) were grown in Ham's F-12K medium (Procell) containing 10% FBS. HEK-293T cells (Zhongqiaoxinzhou, Shanghai, China) were maintained in DMEM (Gibco) containing 10% FBS. All cells were cultured in a humidified atmosphere with 5% CO2 at 37°C.
For transfection, circ_0089823 siRNAs (si-circ_0089823) and their negative control (si-NC), circ_0089823 shRNA (sh-circ_0089823) and its negative control (sh-NC) were transfected into A549 cells, while circ_0089823 over-expression plasmid (OE-circ_0089823) and its negative control (vector) were transfected into PLA-801D cells using Lipofectamine 2000 Reagent (Invitrogen, Carlsbad, CA, USA) according to the protocol. Similarly, microRNA mimics, inhibitors and their negative controls (mimics NC and inhibitor NC) were transfected into A549 cell and PLA-801D cells using Lipofectamine 2000 Reagent. Cells transfected with sh-circ_0089823, sh-NC, OE-circ_0089823 or vector were selected with G418 (350–400 μg/ml; Solarbio, Beijing, China) to obtain stably transfected cell clones.
For transfection, circ_0089823 siRNAs (si-circ_0089823) and their negative control (si-NC), circ_0089823 shRNA (sh-circ_0089823) and its negative control (sh-NC) were transfected into A549 cells, while circ_0089823 over-expression plasmid (OE-circ_0089823) and its negative control (vector) were transfected into PLA-801D cells using Lipofectamine 2000 Reagent (Invitrogen, Carlsbad, CA, USA) according to the protocol. Similarly, microRNA mimics, inhibitors and their negative controls (mimics NC and inhibitor NC) were transfected into A549 cell and PLA-801D cells using Lipofectamine 2000 Reagent. Cells transfected with sh-circ_0089823, sh-NC, OE-circ_0089823 or vector were selected with G418 (350–400 μg/ml; Solarbio, Beijing, China) to obtain stably transfected cell clones.
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