Aluminum hydroxide al oh 3

Ovalbumin (OVA; CAS number: 138831/86/4), hesperedin (CAS number: 520-26-3), and aluminum hydroxide (Al (OH) 3; CAS number: 21645/51/2) were obtained from Sigma Aldrich Chemical Co. (USA).

Diesel particulate matter SRM 2975 was purchased from the National Institute of Standards and Technology (NIST, Gaithersburg, USA). The MLE 12 murine type II pneumocyte cell line was obtained from ATCC (Manassas, VA, USA). The fluorogenic dye 2 ´,7 ´-dichlorodihydrofluorescein diacetate (DCFH-DA), ovalbumin (OVA) and aluminum hydroxide (Al(OH)₃) were purchased from Sigma-Aldrich (St Louis, MO, USA). Primary antibodies were purchased from several vendors: COX-2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), HO-1 (R&D systems, Minneapolis, MN, USA), pSTAT3, pP65, and LC3A/B (Cell Signaling Technology, Danvers, MA, USA), and GAPDH (Merck Millipore, Darmstadt, Germany). Glutaraldehyde, paraformaldehyde, and absolute ethanol were obtained from Merck Millipore, osmium tetroxide (OsO₄) was obtained from Polysciences (Warrington, PA, USA), and 99% pure propylene oxide was obtained from Acros Organics (99% pure, Morris Plains, NJ). ELISA kits for mouse serum IgE were purchased from eBioscience (San Diego, CA, USA).