Charcoal stripped fetal bovine serum

Fulvestrant was purchased from SelleckChem, and PPD was synthesized as previously described.^{32 (link)} Ultrapure polysorbate 80 (H2X, UP80) was purchased from NOF America Corporation. Poly(d,l-lactide-co-2-methyl-2-carboxy-trimethylene carbonate)-graft-poly(ethylene glycol) (PLAC–PEG) was synthesized as previously described.²⁹ CholEsteryl BODIPY FL C12 and 543/563 C11 were purchased from Thermo Fisher Scientific. Charcoal-stripped fetal bovine serum was purchased from Wisent Bio Products. RPMI and DMEM-F12 media were purchased from Sigma-Aldrich. Growth factor-reduced Matrigel was purchased from Corning.

PPD was synthesized from expired clinical samples of doxorubicin (FeRx Inc., Aurora, CO) as previously described.^{27 (link)} Fulvestrant was purchased from Selleckchem. Poly(d,l-lactide-co-2-methyl-2-carboxy-trimethylene carbonate)-graft-poly(ethylene glycol) (PLAC–PEG) was synthesized by ring-opening polymerization using a pyrenebutanol initiator to a molecular weight of 12 000 g/mol and conjugated with an average of three PEG chains/backbone (10 000 g/mol PEG) as previously described.²⁸ Polysorbate 80 (H2X, UP80) was purchased from NOF America Corporation. Vitamin E-PEG 1000 (VitEPEG), Pluronic F68, Pluronic F127, Brij L23, and Brij 58 were purchased from Sigma-Aldrich. McCoy’s 5A cell culture media, CholEsteryl BODIPY 542/563 C11, and Hoechst 33342 were purchased from Thermo Fisher Scientific. The SKOV-3 cell line was purchased from ATCC. Charcoal stripped fetal bovine serum and Hank’s balanced salt solution were purchased from Wisent Bioproducts.