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P s6s236 236

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P-S6S236/236 is a primary antibody product from Cell Signaling Technology. It is used to detect phosphorylation of the S6 ribosomal protein at serine residues 235 and 236.

Automatically generated - may contain errors

Lab products found in correlation

Anti v5 r960 25, by Thermo Fisher Scientific (2 mentions) Rabbit anti akt pt308, by Cell Signaling Technology (2 mentions) Anti ps473 akt, by Cell Signaling Technology (2 mentions) Anti nedd4l 13690 1 ap, by Proteintech (2 mentions) Anti ub sc 8017, by Santa Cruz Biotechnology (2 mentions) Protein assay reagent, by Bio-Rad (2 mentions) Anti pan akt, by Cell Signaling Technology (2 mentions) Anti s6, by Cell Signaling Technology (2 mentions) Phosphatases inhibitors, by Merck Group (2 mentions) Protease inhibitor, by Roche (2 mentions)

2 protocols using p s6s236 236

1

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA buffer (50mM Tris HCl, pH 7.7; 0.15M NaCl; 1mM EDTA; 1% Triton X-100) supplemented with protease inhibitors (Roche) and phosphatases inhibitors (Sigma Aldrich). Protein concentration was measured using Bio-Rad protein assay reagent (Biorad Laboratories, CA). Samples were denatured at 95°C for 10 min in loading buffer then resolved in SDS-PAGE and transferred onto nitrocellulose membranes. Membranes were then blocked in 5% non-fat milk in TBS buffer, 0.1% Tween and then immunoblotted using the following primary antibodies in the specified concentrations: anti-Nedd4l (13690-1-AP, rabbit, Proteintech, 1:1000), anti-Actin (mouse, IGBMC, 1:1000), anti-Akt-pS473 (#4060, rabbit, Cell Signalling, 1:1000), rabbit anti-Akt-pT308 (#2965, rabbit, Cell Signalling, 1:1000) anti-Akt (pan) (#4691, rabbit, Cell Signalling, 1:1000), p-S6S236/236 (#2211, rabbit, Cell Signalling, 1/1000), anti-S6 (#2217, rabbit, Cell Signalling, 1:1000), anti-Ub (sc-8017, mouse, Santa Cruz, 1:250), anti-V5 (R96025, mouse, Invitrogen, 1:5000). All WB experiments consist of at least 3 independent replicates.
Broix L., Jagline H., Ivanova E., Schmucker S., Drouot N., Clayton-Smith J., Pagnamenta A.T., Metcalfe K.A., Isidor B., Louvier U.W., Poduri A., Taylor J.C., Tilly P., Poirier K., Saillour Y., Lebrun N., Stemmelen T., Rudolf G., Muraca G., Saintpierre B., Elmorjani A., Moïse M., Weirauch N.B., Guerrini R., Boland A., Olaso R., Masson C., Tripathy R., Keays D., Beldjord C., Nguyen L., Godin J., Kini U., Nischké P., Deleuze J.F., Bahi-Buisson N., Sumara I., Hinckelmann M.V, & Chelly J. (2016). Mutations in the HECT domain of NEDD4L lead to AKT/mTOR pathway deregulation and cause periventricular nodular heterotopia. Nature genetics, 48(11), 1349-1358.
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2

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA buffer (50mM Tris HCl, pH 7.7; 0.15M NaCl; 1mM EDTA; 1% Triton X-100) supplemented with protease inhibitors (Roche) and phosphatases inhibitors (Sigma Aldrich). Protein concentration was measured using Bio-Rad protein assay reagent (Biorad Laboratories, CA). Samples were denatured at 95°C for 10 min in loading buffer then resolved in SDS-PAGE and transferred onto nitrocellulose membranes. Membranes were then blocked in 5% non-fat milk in TBS buffer, 0.1% Tween and then immunoblotted using the following primary antibodies in the specified concentrations: anti-Nedd4l (13690-1-AP, rabbit, Proteintech, 1:1000), anti-Actin (mouse, IGBMC, 1:1000), anti-Akt-pS473 (#4060, rabbit, Cell Signalling, 1:1000), rabbit anti-Akt-pT308 (#2965, rabbit, Cell Signalling, 1:1000) anti-Akt (pan) (#4691, rabbit, Cell Signalling, 1:1000), p-S6S236/236 (#2211, rabbit, Cell Signalling, 1/1000), anti-S6 (#2217, rabbit, Cell Signalling, 1:1000), anti-Ub (sc-8017, mouse, Santa Cruz, 1:250), anti-V5 (R96025, mouse, Invitrogen, 1:5000). All WB experiments consist of at least 3 independent replicates.
Broix L., Jagline H., Ivanova E., Schmucker S., Drouot N., Clayton-Smith J., Pagnamenta A.T., Metcalfe K.A., Isidor B., Louvier U.W., Poduri A., Taylor J.C., Tilly P., Poirier K., Saillour Y., Lebrun N., Stemmelen T., Rudolf G., Muraca G., Saintpierre B., Elmorjani A., Moïse M., Weirauch N.B., Guerrini R., Boland A., Olaso R., Masson C., Tripathy R., Keays D., Beldjord C., Nguyen L., Godin J., Kini U., Nischké P., Deleuze J.F., Bahi-Buisson N., Sumara I., Hinckelmann M.V, & Chelly J. (2016). Mutations in the HECT domain of NEDD4L lead to AKT/mTOR pathway deregulation and cause periventricular nodular heterotopia. Nature genetics, 48(11), 1349-1358.
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